Depletion of SMN protein in mesenchymal progenitors impairs the development of bone and neuromuscular junction in spinal muscular atrophy

Version of Record

The authors declare this version of their article to be the Version of Record.

Download
Cite
Share
CommentOpen annotations (there are currently 0 annotations on this page).

Version of Record published: February 6, 2024 (This version)
Reviewed preprint version 2: January 23, 2024 (Go to version)
Reviewed preprint version 1: November 7, 2023 (Go to version)
Preprint posted: October 10, 2023 (Go to version)
Sent for peer review: September 21, 2023

1. Of interest
Txnip deletions and missense alleles prolong the survival of cones in a retinitis pigmentosa mouse model

Yunlu Xue, Yimin Zhou, Constance L Cepko

Research Advance May 10, 2024
Further reading

Abstract
eLife assessment
Introduction
Results
Discussion
Methods
Data availability
References
Article and author information
Metrics

Abstract

Spinal muscular atrophy (SMA) is a neuromuscular disorder characterized by the deficiency of the survival motor neuron (SMN) protein, which leads to motor neuron dysfunction and muscle atrophy. In addition to the requirement for SMN in motor neurons, recent studies suggest that SMN deficiency in peripheral tissues plays a key role in the pathogenesis of SMA. Using limb mesenchymal progenitor cell (MPC)-specific SMN-depleted mouse models, we reveal that SMN reduction in limb MPCs causes defects in the development of bone and neuromuscular junction (NMJ). Specifically, these mice exhibited impaired growth plate homeostasis and reduced insulin-like growth factor (IGF) signaling from chondrocytes, rather than from the liver. Furthermore, the reduction of SMN in fibro-adipogenic progenitors (FAPs) resulted in abnormal NMJ maturation, altered release of neurotransmitters, and NMJ morphological defects. Transplantation of healthy FAPs rescued the morphological deterioration. Our findings highlight the significance of mesenchymal SMN in neuromusculoskeletal pathogenesis of SMA and provide insights into potential therapeutic strategies targeting mesenchymal cells for the treatment of SMA.

eLife assessment

This important work by Hann et al. advances our understanding of the role of the survival motor neuron (SMN) protein in coordinating pathogenesis of the spinal muscular atrophy (SMA). The authors addressed many concerns raised by the reviewers, providing convincing evidence in terms of skeletal analyses not being able to satisfactorily elucidate SMN regulation of bone development.

https://doi.org/10.7554/eLife.92731.3.sa0

About eLife assessments

Introduction

The survival motor neuron (SMN) protein is a crucial component of the spliceosome complex and is essential for the proper function of all cell types (Mercuri et al., 2022). Deficiency in SMN protein disrupts the formation of spliceosome complexes, ultimately causing splicing defects in multiple genes. Mutations in the SMN1 gene, which encodes the SMN protein, give rise to the neuromuscular disorder spinal muscular atrophy (SMA). SMA is characterized by neuromuscular junctions (NMJs) disruption, muscular atrophy, and alpha motor neuron loss (Mercuri et al., 2022; Burghes and Beattie, 2009). The severity of the disease in humans correlates with the copy number of SMN2, which is a paralog of SMN1 in humans. SMN2 primarily produces less functional exon 7-deleted SMN protein and rarely generates a limited quantity of functional full-length SMN protein via alternative splicing. SMA patients are classified into types 0 through 4 based on the severity and timing of disease onset, predominantly determined by the number of copies of the SMN2 gene they possess. More than 50% of SMA patients are categorized as type 1, characterized by muscle defects with proximal muscle atrophy during infancy, eventually resulting in death within a few years (Mercuri et al., 2022).

Previous studies suggest that the onset of SMA is mainly attributed to SMN loss in motor neurons (Monani et al., 2000; Burghes and Beattie, 2009). Nevertheless, motor neuron-specific SMN deficiency in the SMA mouse model exhibits relatively mild phenotypes compared to whole-body SMA mouse models (Park et al., 2010; McGovern et al., 2015). Furthermore, restoring SMN to motor neurons in SMA mouse models result in only partial rescue in lifespan and neuromuscular defects (Passini et al., 2010; Martinez et al., 2012; McGovern et al., 2015; Besse et al., 2020). Systemic administration of antisense oligonucleotide (ASO), which corrects SMN2 splicing to restore SMN expression, significantly prolongs survival compared to central nervous system (CNS) administration (Hua et al., 2011). In the mouse treated with a systemically delivered ASO, blocking the effect of ASO in the CNS by a complementary decoy did not have any detrimental effect on survival, motor function, or NMJ integrity (Hua et al., 2015). These studies suggest that peripheral SMN plays a crucial part in SMA pathology. Overall, investigating the impacts of SMN depletion in peripheral tissues is critical for alleviating neuromuscular impairments and increasing life expectancy in SMA.

In SMA patients, bone growth retardation has been observed (De Amicis et al., 2021; Kipoğlu et al., 2019; Hensel et al., 2020). Studies using whole-body SMA mouse models have revealed that this is caused by diminished growth plate chondrocyte density and endochondral ossification defects, independent of muscle atrophy (Hensel et al., 2020). However, it is still unclear whether these defects result from the SMN ablation in bone-forming cells, or from a decline in liver-derived insulin-like growth factor (IGF) in SMA patients and mice (Hua et al., 2011; Yesbek Kaymaz et al., 2016). In severe SMA mice, the serum levels of IGF decreased by approximately 60% or became undetected (Hua et al., 2011; Murdocca et al., 2012). The decreased serum IGF levels were attributed to decreased expression of liver genes, including Igf1, IGF binding, and ternary complex protein gene Igfals and Igfbp3. The previous studies suggest that the liver is the primary origin of systemic IGF, as demonstrated by the liver-specific deletion of Igf1 and the knockout of Igfals, which is mostly expressed in the liver (Yakar et al., 1999; Yakar et al., 2002). The double KO mice exhibited a 90% decrease in serum IGF levels and displayed a phenotype of shortened femur length and growth plates. It is thus possible that the decrease in serum IGF levels, resulting from reduced liver IGF pathway genes in SMA mice, has also played a role in the observed bone growth defect.

Mesenchymal progenitor cells (MPCs) derived from the lateral plate mesoderm (LPM) differentiate into various types of limb mesenchymal cells, including bone, cartilage, and intramuscular mesenchymal cells like fibro-adipogenic progenitors (FAPs) (Nassari et al., 2017). Recent studies have revealed the role of FAPs in skeletal muscle homeostasis, as reducing the number of FAPs resulted in diminished muscle regeneration capacity, long-term muscle atrophy, and NMJ denervation (Wosczyna et al., 2019; Uezumi et al., 2021). Our latest research demonstrated that FAP-specific deficiency of Bap1, one of the deubiquitinases, leads to NMJ defects (Kim et al., 2022). These recent findings raise the possibility that FAPs may have a specific role in the pathogenesis of neuromuscular diseases such as SMA. However, it has not been studied if depletion of SMN in FAPs can lead to SMA-like neuromuscular pathology.

In this study, we crossed a limb MPC-specific Cre mouse with a floxed Smn1 exon 7 mouse carrying multiple copies of the human SMN2 gene, allowing us to examine the impact of mesenchymal SMN reduction on SMA pathogenesis. As a result, the mutant mice showed skeletal growth abnormalities and local IGF signaling defects in the growth plate. In addition, our findings indicate that the SMN reduction in FAPs, similar to the extent of severe SMA, causes altered NMJ development.

Results

Bone growth restriction and growth plate defects caused by MPC-specific SMN depletion

To investigate the effects of SMN reduction within MPCs in SMA pathogenesis, we crossed Smn1^f7/f7 mice, which possess loxP sites flanking exon 7 of the Smn1 gene (Frugier et al., 2000), with Prrx1^Cre mice. This produced Smn1^ΔMPC mice (Prrx1^Cre; Smn1^f7/f7) that lacked the Smn1 gene specifically in Prrx1^Cre-expressed limb MPCs that give rise to bone, cartilage, and FAPs (Logan et al., 2002; Leinroth et al., 2022). To ascertain whether mutant mice carrying the SMN2 gene, like SMA patients, present pathological phenotypes, we additionally generated SMN2 2-copy Smn1^ΔMPC (Prrx1^Cre; Smn1^f7/f7; SMN2^+/+) and SMN2 1-copy Smn1^ΔMPC (Prrx1^Cre; Smn1^f7/f7; SMN2^+/0) mice. Control littermates that lacked Prrx1^Cre were used as controls for comparison.

SMN2 0-copy Smn1^ΔMPC mice died within 24 hr after birth. Regions where limbs should have formed at E18.5 only had rudimentary limb structures (Figure 1—figure supplement 1A). Furthermore, since the upper head bone did not cover the brain, it was directly attached to the skin and protruded. These observations can be attributed to Prrx1^Cre-mediated SMN deletion in the LPM-derived limb MPCs and the craniofacial mesenchyme, which is accountable for the formation of calvarial bone (Wilk et al., 2017). To investigate whether the lack of SMN proteins in MPCs is responsible for bone development abnormalities, we performed alcian blue and alizarin red staining on E18.5 SMN2 0-copy Smn1^ΔMPC mutants to analyze the structure of bones and cartilage. The appendages displayed restricted bone and cartilage formations, with scarcely discernible femur and tibia (Figure 1—figure supplement 1B, C). In the cranial region, there was an absence of both cartilage and bone at the location of the calvarial bone, with the parietal bone entirely missing and partially absent frontal bone (Figure 1—figure supplement 1D). Additionally, the sternum, which is one of the bones originating from the LPM (Sheng, 2015), was shorter than the control (Figure 1—figure supplement 1E).

The SMN2 2-copy Smn1^ΔMPC mice, carrying two homologous SMN2 genes, did not show any discernible differences from the Prrx1^Cre-negative control littermates into adulthood. However, the SMN2 1-copy Smn1^ΔMPC mice exhibited reduced body size and shorter limb length compared to the SMN2 1-copy control (Smn1^f7/f7; SMN2^+/0). To assess postnatal bone growth defects observed in SMA patients and mouse models, we conducted micro-computed tomography (micro-CT) analysis on femurs obtained from postnatal day 14 (P14) SMN2 1-copy Smn1^ΔMPC and SMN2 2-copy Smn1^ΔMPC mice (Figure 1A). The 3D reconstruction image showed that the SMN2 1-copy mutant femur was smaller than the WT control and SMN2 2-copy mutant, and secondary ossification center is denied. The longitudinal virtual section view displayed reduced trabecular bone in the SMN2 1-copy mutant femur. CT analysis data showed that SMN2 1-copy mutants exhibited reduced femur diaphysis length, diameter, and trabecular bone volume compared to the control group, indicating growth plate-dependent endochondral ossification defects (Figure 1B–D). We then examined femoral bone thickness and diaphyseal bone mineral density (BMD) to determine whether mineralization was normal after bone formation. The thickness of the bone in the mutants did not differ significantly from the control, suggesting that bone mineralization was intact (Figure 1E and Figure 1—figure supplement 2A). Unexpectedly, BMD slightly increased in SMN2 1-copy mutants compared to the control group (Figure 1—figure supplement 2B). To assess the impact of osteoclasts and osteoblasts on diaphysis cortical bone mineralization, we utilized Itgb3 immunofluorescence as the osteoclast marker and toluidine blue staining for imaging bone-attached osteoblasts (Romeo et al., 2019; Colaianni et al., 2015). Osteoclast and osteoblast density did not significantly differ between the SMN2 1-copy mutant and the control (Figure 1—figure supplement 2C–E). The higher BMD may be attributed to greater mechanical stress caused by the shorter femur supporting the weight of the body, consistent with prior research indicating that elevated mechanical force leads to higher BMD in the femur (Hoxha et al., 2014; Ike et al., 2015). Nevertheless, the decrease in bone growth without apparent deterioration in bone mineralization of the femur of SMN2 1-copy Smn1^ΔMPC mutants is consistent with findings from the whole-body SMA mouse model (Hensel et al., 2020). Collectively, these results suggest that mice carrying low copies of SMN2, with the Smn1 gene specifically deleted in MPCs, exhibit bone growth abnormalities, while osteoblast and osteoclast populations show no obvious defects based on our preliminary analyses.

Figure 1 with 2 supplements see all

Download asset Open asset

Skeletal growth abnormalities and altered growth plate homeostasis in *SMN2* 1-copy *Smn1^ΔMPC* mice.

(A) Representative 3D images and longitudinal section view of the ossified femur bone. Scale bars, 1 mm. (**B, C**) *SMN2* 1-copy mutant’s femurs showed reduced growth in diaphysis length and diameter, and (D) decreased trabecular bone volume. (E) Trabecular bone thicknesses were not significantly different between the control and mutant groups. The micro-computed tomography (micro-CT) analysis was performed in femur diaphysis and metaphysis from *SMN2* 1-copy *Smn1*^ΔWT, *SMN2* 2-copy, and 1-copy *Smn1^ΔMPC* mice at P14. One-way analysis of variance (ANOVA) with Tukey’s post hoc test, n = 3–5 mice in each genotype (**B–E**). (F) Representative images of hematoxylin and eosin (H&E) staining in the distal femur growth plate of control and mutant mice with 1 copy of *SMN2* at P14. Scale bars, 100 μm. Resting zone (RZ), hypertrophic zone (HZ), and proliferative zone (PZ). (**G–I**) Indicated by black arrows, the HZ and PZ lengths were reduced in *SMN2* 1-copy *Smn1^ΔMPC* mice, and the hypertrophic cell number in a section of the 1-copy mutant was decreased (n = 4 mice in each genotype; unpaired t-test with Welch’s correction). (J) Representative images of Ki67 immunostaining in the distal femur growth plate of control and mutant mice with 1 copy of *SMN2* at P14. Scale bars, 100 μm, and (K) decreased Ki67+ percentage in resting zone chondrocytes. (L) Ki67+ percentage in the proliferative zone was not significantly different between the control and mutant groups. n = 3 mice in each genotype; unpaired t-test with Welch’s correction (**K–L**). ns: not significantly different. *p < 0.05; **p < 0.01. Error bars show standard error of the mean (SEM).

Various bone-forming cells originating from the LPM were known to contribute to bone formation, such as growth plate chondrocytes and osteoblasts. In SMN2 1-copy Smn1^ΔMPC mutants, SMN may be deleted in these cells, suggesting that they play a role in the bone growth abnormalities observed in 2-week-old mice. Previous researchers revealed that primary osteoblasts from a severe SMA mouse model did not display notable differences from controls in an in vitro ossification test. And they did not observe any differences in bone voxel density and bone thickness in femurs at P3 severe SMA mice. This is supported by the absence of any bone thickness or BMD defects in the SMN2 1-copy mutant (Figure 1E and Figure 1—figure supplement 2A, B), and the unimpaired osteoblast population (Figure 1—figure supplement 2E). Thus, we conclude that the bone growth abnormalities observed in the 2-week-old SMN2 1-copy Smn1^ΔMPC mutant are due to impaired endochondral ossification.

To determine whether bone growth defects in SMN2 1-copy Smn1^ΔMPC mutants arise from disrupted chondrocyte homeostasis at growth plates, we stained the femur distal growth plate of P14 mice with hematoxylin and eosin (H&E; Figure 1F). In line with earlier findings in whole-body SMA mice (Hensel et al., 2020), SMN2 1-copy Smn1^ΔMPC mice exhibited shorter proliferative and hypertrophic zones compared to control mice (Figure 1G, H). Additionally, there was a significant reduction in the number of chondrocytes in the hypertrophic zone (Figure 1I). To investigate the decrease in chondrocyte proliferation and subsequent reduction in the proliferative and hypertrophic zone, we stained the proliferation marker Ki67 in the growth plate of both SMN2 1-copy control and mutant samples (Figure 1J). We then quantified the percentage of Ki67+ nuclei in the resting and proliferative zones (Figure 1K, L). Although there was no significant difference observed in the Ki67+ percentage of proliferative zone chondrocytes in the proceeding proliferation state, there was an absolute reduction in resting zone chondrocyte proliferation. The decreased proliferation rate in the resting zone could have impeded the transition to the proliferative zone. Our data indicate that adequate expression of SMN is essential for the homeostasis of chondrocytes at growth plates.

Disruption of chondrocyte-derived IGF signaling in SMN2 1-copy Smn1^ΔMPC mutants

The proliferation and differentiation of growth plate chondrocytes are regulated by systemic IGF (Shim, 2015; Karimian et al., 2011; Racine and Serrat, 2020). Previous research suggested that a key factor contributing to the pathological phenotype in SMA is the lowered expression of the Igf1/Igfbp3/Igfals genes, which produce IGF and IGF-carrying proteins, in the liver (Hua et al., 2011; Murdocca et al., 2012). As the IGF pathway proteins are downregulated in whole-body SMA mice, the bone growth defects observed in the mice have sparked debate as it remains unclear whether they are due to cell-autonomous defects by bone-forming cells’ SMN reduction, or the low liver-derived IGF level (Hua et al., 2011; Tsai et al., 2014; Deguise et al., 2021; Hensel et al., 2020).

To clarify this issue, we used Smn1^ΔMPC mutant mice, which enabled us to investigate the effect of SMN depletion in bone-forming cells such as chondrocytes on bone growth, while circumventing the impact of the endocrine signal by Prrx1-negative organ. To investigate the impact of IGF signaling on growth plate chondrocytes, we employed immunofluorescence to evaluate the percentage of p-AKT-positive cells activated by the IGF–PI3K–AKT pathway in both SMN2 1-copy control and mutant femur distal growth plate (Figure 2A). Intriguingly, the percentage of p-AKT+ cells was significantly decreased in resting zone chondrocytes, but not in the proliferative zone, which aligns with the Ki67+ percentage (Figure 2B, C). Expectedly, the liver’s mRNA expression of IGF pathway genes, reported to be decreased in SMA mouse models and patients (Hua et al., 2011; Murdocca et al., 2012; Deguise et al., 2021; Sahashi et al., 2013), showed no difference when comparing controls to SMN2 1-copy Smn1^ΔMPC mutants (Figure 2D). These findings indicate that the growth plate’s proliferation and hypertrophy in SMN2 1-copy mutants are affected by impairments in another AKT upstream signal rather than by liver-secreted systemic IGF.

Figure 2

Download asset Open asset

Decreased chondrocyte-derived IGF–AKT axis by limb mesenchymal cell-specific survival motor neuron (SMN) depletion in *SMN2* 1-copy *Smn1^ΔMPC* mice.

(A) Representative images of p-AKT immunostaining in distal femur growth plate from mice at P14. Scale bars, 100 μm. (**B, C**) The p-AKT-positive percentage was decreased in the resting zone chondrocytes, not in the proliferative zone (n = 3–4 mice in each genotype; unpaired t-test with Welch’s correction). (D) Relative IGF axis mRNA expression in the livers of *SMN2* 1-copy control and mutant mice. The IGF pathway genes showed no difference when comparing controls to *SMN2* 1-copy *Smn1^ΔMPC* mutants. (**E, F**) Relative IGF axis and chondrocyte differentiation marker mRNA expression in the chondrocytes of *SMN2* 1-copy control and mutant mice. The *Igf1*, *Igfbp3*, and hypertrophic marker *Col10a1* expression were decreased in *SMN2* 1-copy *Smn1^ΔMPC* mutants. n = 3 mice in each genotype; unpaired t-test with Welch’s correction (**D–F**). (G) Representative images of genomic PCR analysis from *SMN2* 1-copy *Smn1^ΔMPC* mice tissues at P21. (H) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis from tissues of *SMN2* 1-copy *Smn1^WT* and *Smn1^ΔMPC* mice at P21 (n = 3 mice in each genotype; unpaired t-test with Welch’s correction). Deletion of *Smn1* exon 7 was detected only in limb mesenchymal cells using genomic PCR (G) and full-length *Smn1* mRNA expression (H). (I) Representative images of western blot analysis in cultured fibro-adipogenic progenitors (FAPs). SMN protein in FAPs of *SMN2* 1-copy *Smn1^ΔMPC* mice exhibited a decrease comparable to that observed in the SMAΔ7 mice. (J) Relative SMN levels in cultured FAPs of the controls and mutants (n = 3 mice in each genotype; one-way analysis of variance (ANOVA) with Tukey’s post hoc test). ns: not significantly different. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.001. Error bars show standard error of the mean (SEM).

Figure 2—source data 1 Original file for the gel electrophoresis of genomic PCR in Figure 2G (Cre, Smn1^F7, Smn1^Δ7) and western blot analysis in Figure 2I (anti-alpha-tubulin, anti-SMN).: https://cdn.elifesciences.org/articles/92731/elife-92731-fig2-data1-v1.zip
Download elife-92731-fig2-data1-v1.zip
Figure 2—source data 2 PDF containing Figure 2G, I and original scans of the PCR and western blot with highlighted bands and sample labels.: https://cdn.elifesciences.org/articles/92731/elife-92731-fig2-data2-v1.zip
Download elife-92731-fig2-data2-v1.zip

There are reports indicating that local IGF expression plays a crucial role in bone development through the growth plate, in addition to circulating IGF (Hallett et al., 2019; Racine and Serrat, 2020). While most Igf1-null mice died before birth and had smaller tibial lengths than normal, liver-specific Igf1-deleted mice did not experience significant changes in body length or tibial length during postnatal growth, despite a 75% reduction in serum IGF-1 levels (Baker et al., 1993; Yakar et al., 1999). This indicates that local IGF in the growth plate is crucial for endochondral ossification, in addition to serum IGF. The chondrocyte-specific Igf1 knockout mouse demonstrated a reduction in postnatal body and femur length, and the chondrocyte-specific Igf1r knockout mouse demonstrated a significant reduction in bone growth, as well as a decrease in both growth plate proliferative and hypertrophic zone (Govoni et al., 2007; Wang et al., 2011). A recent study revealed that resting zone chondrocytes in the growth plate serve as a major source of local IGF and activate the p-AKT pathway via autocrine and paracrine IGF signaling (Oichi et al., 2023). The study further revealed that cells that constitute bone and bone marrow, apart from chondrocytes, do not express Igf1, making chondrocytes the solitary source of local IGF. These suggest that the growth plate defects and the reduction of resting zone AKT phosphorylation in SMN2 1-copy mutants may be due to chondrocyte-secreted IGF deficiency. To confirm this hypothesis, we evaluated the expression of IGF-related genes in chondrocytes from both SMN2 1-copy control and mutant femur (Figure 2E). Indeed, Igf1 and Igfbp3 were greatly depleted in SMN2 1-copy mutant chondrocytes. It is hypothesized that the increased presence of Ghr may be in response to the reduction of Igf1. As IGF directly causes chondrocyte hypertrophy (Wang et al., 1999), we assess the mRNA expression of chondrocyte hypertrophic marker Col10a1 and undifferentiated chondrocyte marker Col2a1 (Figure 2F). The results show that Col10a1 is decreased, while Col2a1 is increased in the mutant. In Figure 1H, I, the hypertrophic cell reduction may be caused by a low local IGF level. Therefore, depletion of local IGF in the growth plate of SMN2 1-copy mutants may hinder chondrocyte progression to proliferation and hypertrophy, leading to aberrations in endochondral ossification. In Figures 1L and 2C, it is possible that the serum IGF could affect the remnant proliferative and p-AKT-positive cells in the growth plate via the vascularization of bone marrow. Based on these findings, it was concluded that deprivation of SMN in chondrocytes leads to a decrease in local IGF signaling, which affects growth plate homeostasis.

Mesenchymal cell-specific SMN reduction similar to severe SMA mouse model in SMN2 1-copy Smn1^ΔMPC mutants

To confirm the specific deletion of Smn1 in limb mesenchymal cells, including chondrocytes and FAPs, we performed quantitative reverse transcription polymerase chain reaction (qRT-PCR) for full-length Smn1 mRNA expressed by undeleted Smn1 allele. Our findings indicate that full-length Smn1 mRNA expression in the brain, liver, skeletal muscle, and spinal cord of SMN2 1-copy Smn1^ΔMPC mice at postnatal day 21 (P21) was similar to that of control mice, while it was significantly reduced in isolated FAPs and chondrocytes (Figure 2H). Additionally, we confirmed the presence of the Smn1^Δ7 variant, which is the exon 7-deleted form of Smn1 created by cre-lox-mediated recombination, in both FAPs and chondrocytes by conducting genomic PCR on the SMN2 1-copy Smn1^ΔMPC mutant (Figure 2G). Since SMN was not downregulated in the tissues other than limb mesenchymal cells, non-mesenchymal cells were ruled out from being responsible for the phenotype observed in Smn1^ΔMPC mutants.

Among our Smn1^ΔMPC models, mice with two copies of SMN2 exhibit similar bone development parameters as control mice without SMN deletion. However, mice with one copy of SMN2 display bone pathological defects akin to SMA mouse models. This may be because the quantity of human full-length SMN protein produced by SMN2 2-copy, was sufficient to sustain SMN complex function in the SMN2 2-copy Smn1^ΔMPC mutants, despite the absence of functional mouse SMN protein in limb mesenchymal cells. On the contrary, due to the lower expression of full-length SMN compared to SMN2 2-copy mutants, the SMN complex may not function properly in SMN2 1-copy mutant cells. We confirmed this by comparing the amount of full-length SMN protein in isolated FAPs from the hindlimbs of control, Smn1^ΔMPC mutants, and a severe SMA mouse model (Smn1^−/−; SMN2^+/+; SMNΔ7^+/+; SMAΔ7 mutants) (Figure 2I). The data show that SMN2 1-copy Smn1^ΔMPC mutants exhibited ~80% reduction in SMN levels compared to the control group. The level of SMN protein in SMN2 1-copy Smn1^ΔMPC mutants was similar to that in SMAΔ7 mutants. Conversely, SMN2 2-copy mutants display a decrease of approximately 40% in SMN protein levels compared to the control (Figure 2J). The moderately reduced expression of SMN is adequate to support regular bone development in SMN2 2-copy mutants. Taken together, our findings indicate that the reduction of mesenchymal SMN to levels comparable to that of the severe SMA mouse model causes SMA-like bone pathology in the SMN2 1-copy mutant.

Abnormal NMJ maturation in SMN2 1-copy Smn1^ΔMPC mutants

To investigate whether disabling SMN in FAPs results in SMA-like neuromuscular impairments, we assessed if NMJ phenotypes observed in SMA mouse models also occur in SMN2 1-copy Smn1^ΔMPC mutants. Both severe and mild SMA mouse models exhibit impaired NMJ maturation markers, including plaque-like morphology of acetylcholine receptor (AChR) clusters, neurofilament (NF) varicosities, and poor terminal arborization (Kong et al., 2009; Martinez et al., 2012; Monani et al., 2003; Kariya et al., 2008). To evaluate the impact of SMN deficiency in FAPs on NMJ maturation, we evaluated the NMJ maturation markers in the tibialis anterior (TA) muscles of control and SMN2 1-copy Smn1^ΔMPC mutant mice at P21, a time when NMJ maturation is in progress (Figure 3A). Our examination revealed the presence of NF varicosities in SMN2 1-copy mutants as compared with control mice (Figure 3A, D). Additionally, the number of nerve branches was decreased and half of the total NMJs were poorly arborized in SMN2 1-copy mutants (Figure 3B, C). These presynaptic alterations are specific phenotypes in neurogenic atrophy-like SMA. Unlike neurogenic atrophy, physiologic atrophy shows no differences in presynaptic morphology, such as nerve branching (Deschenes et al., 2006). This suggests that the NMJ phenotypes observed in SMN2 1-copy Smn1^ΔMPC mutant mice are not caused by decreased muscle size and activity resulting from bone growth abnormalities. The morphology of AChR clusters shows that the mutants have more immature plaque-like NMJs than the controls’ pretzel-like structure (Figure 3E). Therefore, these findings indicate that SMN2 1-copy Smn1^ΔMPC mutants exhibit NMJ maturation abnormalities common in SMA mouse models.

Figure 3

Download asset Open asset

Aberrant postnatal neuromuscular junction (NMJ) maturation in *SMN2* 1-copy *Smn1^ΔMPC* mice.

(A) Immunostaining of NMJs in TA muscle of *SMN2* 1-copy *Smn1^WT* and *Smn1^ΔMPC* mice at P21 with anti-NF (green), anti-synaptophysin (blue), and α-Btx staining acetylcholine receptor (AChR; red). Scale bars, 20 μm. The confocal images of NMJs showed decreased presynaptic terminal branching and the existence of nerve terminal varicosities that were enlarged with neurofilament (NF; indicated by arrowheads) in the mutant. (B) The NMJs of the *SMN2* 1-copy mutant exhibited a significant decrease in presynaptic terminal arborization and (C) an increased percentage of poorly arborized NMJs (n = 3 mice in each genotype; unpaired t-test with Welch’s correction). (D) The percentage of NMJs exhibiting NF varicosities was higher in the *SMN2* 1-copy mutant group than in the control group (n = 3–4 mice in each genotype; unpaired t-test with Welch’s correction). (E) For quantification of the NMJ maturation stage, we classified NMJs into five distinct developmental stages (Plaque: plaque-shaped endplate without any perforation; Small perforated: plaque-shaped endplate with small perforations; Large perforated: plaque-shaped endplate with large perforations; Open: C-shaped endplate; Pretzel: pretzel-like shaped endplate) and then compared the frequency patterns of *SMN2* 1-copy control and mutant mice (n = 3 mice in each genotype; two-way analysis of variance (ANOVA) with Tukey’s post hoc test). The NMJs of *SMN2* 1-copy mutants displayed plaque-like shapes, indicating that they were in the immature stage. (F) Immunostaining of NMJs in TA muscle of *SMN2* 1-copy *Smn1^WT* and *Smn1^ΔMPC* mice at P3 with anti-NF (green), anti-synaptophysin (blue), and α-Btx staining AChR (red). Scale bars, 10 μm. (G) There were no significant differences in AChR cluster size between the *SMN2* 1-copy control and mutant at P3 (n = 3–4 mice in each genotype; unpaired t-test with Welch’s correction). (H) The ratio of the Synaptophysin area to the AChR area in NMJ was slightly higher in the *SMN2* 1-copy mutant at P3 (n = 3–4 mice in each genotype; unpaired t-test with Welch’s correction). ns: not significantly different. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. All box-and-whisker plots show the median, interquartile range, minimum, and maximum. For the box-and-whisker plots, range bars show minimum and maximum (**B, G, H**). For the bar and line graph, error bars show standard error of the mean (SEM) (**C–E**).

Undisturbed NMJ formation in neonatal SMN2 1-copy Smn1^ΔMPC mutants

To determine whether any NMJ defects were present prior to juvenile NMJ maturation in SMN2 1-copy Smn1^ΔMPC, we examined NMJ formation in SMN2 1-copy Smn1^ΔMPC mice at the neonatal stage on postnatal day 3. We evaluated the AChR and nerve terminal areas to assess post- and presynaptic development, respectively (Figure 3F). Measurements of AChR cluster size indicated no differences between control and SMN2 1-copy Smn1^ΔMPC mice (Figure 3G). However, the area of AChR covered by nerve terminals was slightly larger in SMN2 1-copy Smn1^ΔMPC (Figure 3H). We have no reasonable explanation for why the coverage is higher in the mutant. However, there does not appear to be abnormal development of the NMJ in the mutant, at least until the neonatal period. Therefore, we reasoned that SMN2 1-copy Smn1^ΔMPC mutants began to exhibit deterioration in the NMJ maturation during the juvenile stage, following the intact neonatal development of NMJ.

Aberrant NMJ morphology in the adult SMN2 1-copy Smn1^ΔMPC mice

To evaluate the organization of NMJ after the conclusion of postnatal NMJ development, considering mesenchymal SMN expression, we examined NMJ morphology in the TA muscle of control, SMN2 1-copy, and 2-copy Smn1^ΔMPC mice at postnatal day 56 (P56). Our analysis revealed that presynapses were fragmented in SMN2 1-copy mutants, resulting in a bouton-like morphology, in contrast to the control and SMN2 2-copy Smn1^ΔMPC mice (Figure 4A). In SMN2 1-copy Smn1^ΔMPC mice, a twofold presynaptic fragmentation compared to the control was quantified, demonstrating nerve terminal shrinkage (Figure 4B). Additionally, NF ends displayed more severe varicosities than at P21 and were only connected to the proximal nerve by very thin NF, unlike control and SMN2 2-copy mice (Figure 4C). Remarkably, numerous presynaptic islands formed in SMN2 1-copy Smn1^ΔMPC mice through the merging of fragmented presynapses and NF varicosity. In SMN2 1-copy mutants, AChR clusters displayed fragmented grape-shaped morphology that overlapped with nerve terminals, whereas control and SMN2 2-copy mice displayed pretzel-like structures (Figure 4D). These results suggest that defects in adult NMJ morphology occur when mesenchymal SMN protein is reduced to the extent of the SMN2 1-copy Smn1^ΔMPC mutants.

Figure 4

Download asset Open asset

Morphological deterioration in neuromuscular junctions (NMJs) of adult *SMN2* 1-copy *Smn1^ΔMPC* mice.

(A) Immunostaining of NMJs in TA muscle of *SMN2* 1-copy *Smn1^WT*, *SMN2* 2-copy, and *SMN2* 1-copy *Smn1^ΔMPC* mice at P56 with anti-NF (green), anti-synaptophysin (blue), and α-Btx staining acetylcholine receptor (AChR; red). Scale bars, 20 μm. The confocal images of NMJs showed fragmentation and bouton-like neurofilament (NF) varicosities (indicated by arrowheads) in the *SMN2* 1-copy *Smn1^ΔMPC* mice. The NMJs of the *SMN2* 1-copy mutant displayed fragmented presynapse (B), endplate (D), and NF varicosities (C) compared to *SMN2* 1-copy *Smn1^WT* and *SMN2* 2-copy *Smn1^ΔMPC* mice (n = 3–5 mice in each genotype; Presynaptic fragments and AChR fragments: Brown–Forsythe and Welch analysis of variance (ANOVA) with Games–Howell’s test; NF varicosities: one-way ANOVA with Tukey’s post hoc test). ns; not significantly different. ****p < 0.0001. All box-and-whisker plots show the median, interquartile range, minimum, and maximum. For the box-and-whisker plots, range bars show minimum and maximum (**B, D**). For the bar graph, error bars show standard error of the mean (SEM) (C).

Presynaptic neurotransmission alteration in SMN2 1-copy Smn1^ΔMPC mutants

To investigate whether the morphologically aberrant NMJs of SMN2 1-copy Smn1^ΔMPC mice have functional impairments, we isolated hindlimb extensor digitorum longus (EDL) muscles from P56 mice and conducted electrophysiological recording. We incubated the muscles with μ-conotoxin, which selectively inhibits muscle voltage-gated Na⁺ channels, prevented the induction of muscle action potential (Ling et al., 2010; Zanetti et al., 2018), and recorded the Miniature endplate potential (mEPP) and evoked endplate potential (eEPP) (Ling et al., 2010; Zanetti et al., 2018). mEPP, a response that occurs when spontaneously released acetylcholine binds to nicotinic AChR without nerve stimulation, was measured ex vivo near the NMJs of the EDL muscles in the control and SMN2 1-copy Smn1^ΔMPC mice (Figure 5A). The mEPP amplitude was increased in SMN2 1-copy Smn1^ΔMPC mice (Figure 5B), whereas mEPP frequency was comparable between the controls and mutants (Figure 5C). The results indicate that the NMJ synapses of SMN2 1-copy Smn1^ΔMPC mice are functional and more sensitive to acetylcholine compared to the controls. Next, we measured the eEPP by stimulating an action potential at the peroneal nerve (Figure 5D). Despite the increased mEPP amplitude, the amplitude of eEPPs was significantly decreased in SMN2 1-copy Smn1^ΔMPC mice (Figure 5E). These results suggest that the nerve terminals in SMN2 1-copy Smn1^ΔMPC mice exhibit decreased quantal content. This could be due to a decrease in vesicle release probability. Notably, there was no difference in the paired-pulse response, indicating normal neurotransmitter release probability (Figure 5F, G). Taken together, these findings suggest that the presynaptic neurotransmission ability of the NMJ is reduced in SMN2 1-copy Smn1^ΔMPC mutants.

Figure 5

Download asset Open asset

Reduced presynaptic neurotransmission ability in the neuromuscular junctions (NMJs) of *SMN2* 1-copy *Smn1^ΔMPC* mice.

(A) Representative traces of Miniature endplate potential (mEPP) from *SMN2* 1-copy *Smn1*^ΔWT (top) and *SMN2* 1-copy *Smn1^ΔMPC* (bottom) mice. (**B, C**) *SMN2* 1-copy mutant’s NMJs showed an increase in mEPP amplitude and no differences in mEPP frequency (1-copy control, n = 25, 9 mice; 1-copy mutant, n = 21, 8 mice; unpaired t-test with Welch’s correction). (D) Representative traces of evoked endplate potential (eEPP) from *SMN2* 1-copy *Smn1*^ΔWT (top) and *SMN2* 1-copy *Smn1^ΔMPC* (bottom) mice. (E) The mutant’s NMJs showed a stronger amplitude of eEPPs (1-copy control, n = 12, 4 mice; 1-copy mutant, n = 12, 3 mice; unpaired t-test with Welch’s correction). (F) Representative traces of paired-pulse response from *SMN2* 1-copy *Smn1*^ΔWT (top) and *SMN2* 1-copy *Smn1^ΔMPC* (bottom) mice. (G) Paired-pulse response was not different between *SMN2* 1-copy control and mutant NMJs, indicating a comparable neurotransmitter release probability (1-copy control, n = 8, 3 mice; 1-copy mutant, n = 6, 3 mice; unpaired t-test with Welch’s correction). The electrophysiological recording was performed in the extensor digitorum longus (EDL) muscle at P56. ns: not significantly different. *p < 0.05. All box-and-whisker plots show the median, interquartile range, minimum, and maximum. For the box-and-whisker plots, range bars show minimum and maximum (**B, C, E, G**).

Disturbed nerve terminal structure in SMN2 1-copy Smn1^ΔMPC mice

To examine the NMJ ultrastructure of SMN2 1-copy Smn1^ΔMPC mutants, we utilized transmission electron microscopy (TEM) (Figure 6A). The density of junctional folds in SMN2 1-copy Smn1^ΔMPC mutant specimens was comparable to that of the control (Figure 6B). However, the density of synaptic vesicles was substantially elevated in the SMN2 1-copy mutants (Figure 6C). Since previous electrophysiological results suggested a decrease in presynaptic neurotransmission capacity in SMN2 1-copy mutants, this could be due to synaptic vesicles failing to fuse with the membrane, leading to the accumulation of vesicles in the terminal and reduced quantal contents in Figure 5. In Figure 3H, larger synaptophysin coverage in the mutant may be caused by this synaptic vesicle accumulation. Additionally, the detachment of the nerve terminal is more frequent at the NMJ of mutants (Figure 6D). The detachment of nerve terminals observed in SMN2 1-copy Smn1^ΔMPC mutants could have also resulted in diminished presynaptic neurotransmission capacity. Collectively, these findings indicate that SMN2 1-copy Smn1^ΔMPC mutants have nerve terminal-specific pathological defects at the NMJ ultrastructural level.

Figure 6

Download asset Open asset

Abnormal nerve terminal ultrastructure in *SMN2* 1-copy mutant.

(A) Representative transmission electron microscopy (TEM) images from neuromuscular junctions (NMJs) of *SMN2* 1-copy *Smn1*^ΔWT and *SMN2* 1-copy *Smn1^ΔMPC* mice at P56. Scale bars, 500 nm. Nerve terminal (NT; indicated by the blue zone). Synaptic vesicles (SVs). Muscle fiber (MS; indicated by the red zone). Endplate junctional folds (JF). Nerve terminal detachment (indicated by arrow) was observed in *SMN2* 1-copy *Smn1^ΔMPC* mice. (B) The density of junctional folds in the NMJ of *SMN2* 1-copy *Smn1^ΔMPC* mice no significant change compared to the control, whereas (C) the density of synaptic vesicles was increased (n = 3–4 mice in each genotype; unpaired t-test with Welch’s correction). (D) The detachment of the nerve terminal occurs more frequently at the NMJ of mutants (n = 3–4 mice in each genotype; unpaired t-test with Welch’s correction). ns: not significantly different. *p < 0.05; ***p < 0.001. All box-and-whisker plots show the median, interquartile range, minimum, and maximum. For the box-and-whisker plots, range bars show minimum and maximum (**B, C**). For the bar graph, error bars show standard error of the mean (SEM) (D).

FAPs transplantation rescues NMJ morphology in limb mesenchymal SMN mutants

SMN-deleted limb mesenchymal tissues in SMN2 1-copy Smn1^ΔMPC mutants comprise not only FAPs, but also bone, cartilage, pericytes, and tendon, among others (Leinroth et al., 2022; Nassari et al., 2017). To evaluate the critical role of FAPs in the postnatal development of the NMJ, we isolated fluorescent protein-labeled wild-type FAPs from Prrx1^Cre; Rosa26^LSL-YFP/+ or Prrx1^Cre; Rosa26 ^{LSL-tdTomato/+} mice and transplanted them into the TA muscles of SMN2 1-copy Smn1^ΔMPC mice at postnatal day 10. The TA muscle on the contralateral side was treated with a vehicle as a control. In SMN2 1-copy Smn1^ΔMPC mice at P56, the muscle that received FAPs showed decreased presynaptic fragmentation, NF varicosities, and postsynaptic fragmentation compared to the contralateral muscle (Figure 7A–D). As a result, the transplanted muscle was not significantly different from the control except for NF varicosities. These data demonstrate that the transplantation of wild-type FAPs rescues the abnormal NMJ development in SMN2 1-copy Smn1^ΔMPC mice. Overall, our findings indicate that SMN depletion in FAPs leads to the neuronal SMN-independent NMJ pathology in severe SMA, which is rescued by the transplantation of healthy FAPs.

Figure 7

Download asset Open asset

Improved postnatal neuromuscular junction (NMJ) development in the TA muscle of *SMN2* 1-copy *Smn1^ΔMPC* mice following healthy fibro-adipogenic progenitors (FAPs) transplantation.

(A) Immunostaining of NMJs in tdTomato⁺ FAP-transplanted TA muscle (+FAP) and vehicle-treated contralateral muscle (+Veh) in *SMN2* 1-copy *Smn1^ΔMPC* mice at P56 with anti-NF (yellow), anti-synaptophysin (blue), α-Btx staining acetylcholine receptor (AChR; green), and tdTomato fluorescence (red). Scale bars, 40 μm. The images revealed neurofilament (NF) varicosities (indicated by arrowheads) in the +Veh NMJs. The tdTomato⁺ FAPs (marked by asterisks) were transplanted into +FAP NMJs, which exhibited (B) decreased presynaptic fragmentation, (C) NF varicosities, and (D) AChR fragmentation compared to +Veh NMJs and similar to wild-type NMJs (n = 3–4 mice in each group; unpaired t-test with Welch’s correction). ns: not significantly different. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All box-and-whisker plots show the median, interquartile range, minimum, and maximum. For the box-and-whisker plots, range bars show minimum and maximum (**B, D**). For the bar graph, error bars show standard error of the mean (SEM) (C).

Discussion

In this paper, we elucidate the contribution of SMN depletion in mesenchymal progenitors for the pathogenesis of SMA. To test this hypothesis, we generated conditional knockout mouse strains to delete the Smn1 allele specifically in limb mesenchymal cells and carry human SMN2 copies. Our research using these mouse models resulted in three major discoveries. First, SMN deficiency in FAPs contributes to NMJ pathological defects in SMA. We observed delayed NMJ maturation and varicosities in juvenile SMN2 1-copy Smn1^ΔMPC mutant. The pathogenic NMJ phenotypes were also observed in the SMAΔ7 mutant, which is one of the severe SMA mouse models (Kong et al., 2009; Martinez et al., 2012; Kariya et al., 2008). As the SMAΔ7 mutant typically lives for approximately 12 days, the fragmentation of the NMJ in adult SMN2 1-copy mutant was not evaluated in the severe SMA mutant. Nevertheless, models that induce a conditional adult SMN deficiency through either Cre^ER allele or oligonucleotide administration resulting in SMN reduction, demonstrated the depletion of SMN throughout the body caused fragmentation of NMJ (Sahashi et al., 2013; Kariya et al., 2014). Thus, we demonstrate that the SMN2 1-copy Smn1^ΔMPC mutant model mimics whole-body SMA mouse models in NMJ morphology. In the electrophysiological test, the SMAΔ7 mutant exhibited reduced quantal content, readily releasable pool, and vesicle release probability (Torres-Benito et al., 2011). In our study, we did not directly assess the readily releasable pool in SMN2 1-copy Smn1^ΔMPC mutant by stimulus train of electrophysiological recording but instead showed reduced quantal content and normal vesicle release probability. In previous studies, it was theorized that the decrease in quantal contents in SMAΔ7 mutants resulted from decreased synaptic vesicle density at nerve terminals caused by motor neuron defects and abnormal axonal transport (Dale et al., 2011; Kong et al., 2009). However, we found that synaptic vesicle density was increased in the SMN2 1-copy Smn1^ΔMPC mutant with SMN-sufficient motor neurons. It is possible that the alteration of active zones, which were also altered in the SMA motor terminals (Kong et al., 2009), contributed to the reduction in synaptic vesicle fusion and the decreased quantal contents. Indeed, nerve terminal detachment in SMN2 1-copy mutant mice was also found in active zone complex protein integrin-α3 knockout mice (Ross et al., 2017). Based on the rescue data of transplanted healthy FAPs, we can report that FAP-specific SMN depletion is involved in NMJ pathology of SMA.

Second, we demonstrated that skeletal growth defects, a phenotype observed in SMA (Khatri et al., 2008; Vai et al., 2015; Wasserman et al., 2017; Baranello et al., 2019; Hensel et al., 2020), are a cell-autonomous pathological effect of the depletion of SMN in bone-forming cells. We observed reduced bone size and volume in juvenile SMN2 1-copy Smn1^ΔMPC mutant. Depletion of SMN in chondrocytes showed growth plate homeostasis problems with chondrocyte-secreted IGF defects. However, our skeletal study has a limitation in that we did not assess potential impairment of endochondral ossification during embryonic development. It may be necessary to further evaluate the embryonic period in addition to the postnatal growth plate defects observed in our study, as cells of the Prrx1-lineage are involved in mesenchymal condensation and chondrocyte differentiation during embryonic endochondral ossification (Hallett et al., 2019; Racine and Serrat, 2020). Previous studies have reported that IGF1 overexpression improves biochemical and behavioral manifestations in SMA mice, suggesting potential therapies for SMA (Bosch-Marcé et al., 2011; Tsai et al., 2014). Our study showed that these IGF therapies for SMA could be one to consider for treating bone growth abnormalities. In addition, the skeletal growth defect may affect physiologic muscle atrophy through imbalanced muscle contraction and reduced tension. This physiologic atrophy may be part of SMA-associated muscle weakness independent of neurogenic atrophy.

Third, it was demonstrated that adequate levels of SMN protein are essential for MPCs to contribute to limb neuromusculoskeletal development. The mutants with only 1 copy of SMN2 exhibit problematic symptoms observed in both SMA patients and mouse models, while SMN2 2-copy mutants display a typical phenotype in bone and NMJ. The lack of SMN protein in MPCs by insufficient SMN2 copies, similar to the deficiency seen in severe SMA, is responsible for the onset of SMA pathology. Thus, we propose that restoration of deficient SMN in MPCs is crucial for rehabilitating their function. Based on these discoveries, SMN replenishment treatments for MPCs, specifically FAPs, and chondrocytes, are necessary to provide a complete solution for neuromusculoskeletal defects in severe SMA patients.

The initial focus for the treatment of SMA was on motor neurons located in the spinal cord, and pharmaceuticals were created to address the deficiency of SMN in these neurons (Mercuri et al., 2020). For example, intrathecal injections of Spinraza can efficiently boost SMN in the CNS, including the spinal cord (Passini et al., 2011; Claborn et al., 2019). However, Spinraza does not address the lack of SMN in peripheral tissues, including mesenchymal cells, highlighted in recent studies. The drug Zolgensma, which employs AAV9 to express SMN through systemic delivery, appears to resolve this issue (Foust et al., 2009; Valori et al., 2010; Mattar et al., 2013). Nevertheless, a prior study indicates that chondrocytes within the growth plate and articular cartilage do not get infected with AAV9 (Yang et al., 2019). Furthermore, it is well established that DNA vectors delivered via AAV9 undergo dilution during cell proliferation (Penaud-Budloo et al., 2008; Colella et al., 2018; Van Alstyne et al., 2021; Heller et al., 2021). Given the infection issue and the dilution issue by the active cell population changes of chondrocytes and FAPs during postnatal development (Petrany et al., 2020; Bachman and Chakkalakal, 2022), SMN supplementation through Zolgensma alone would not be sufficient for severe SMA patients. Thus, there is an urgent need to research and develop therapeutic strategies that target mesenchymal progenitors.

While this study is the first to demonstrate the impact of SMN depletion in FAPs on the NMJ development, it does not elucidate the specific mechanism by which FAPs influence the NMJ. Our study observed NMJ recovery in the muscles transplanted with healthy FAPs, but not in the contralateral muscles, indicating that FAPs are likely involved in NMJ development through juxtacrine or paracrine signaling. Since FAPs interact with surrounding tissues through a variety of signaling factors, such as extracellular matrix (Contreras et al., 2021; Scott et al., 2019), Wnt-related protein (Lukjanenko et al., 2019), and Bmp signaling protein (Uezumi et al., 2021; Camps et al., 2020), mis-splicing of the signaling factors due to SMN reduction could disturb the homeostasis of neighboring tissues (Zhang et al., 2008). Furthermore, the Hsd11b1-positive subpopulation of FAPs associated with NMJ was discovered through single-cell RNA sequencing in a recent study (Leinroth et al., 2022). This population is located adjacent to the NMJ and responds to denervation, indicating an increased possibility of interaction with the NMJ organization. Therefore, it is necessary to conduct additional investigations into the expression of various signaling factors by diverse FAP subpopulations in future studies.

Methods

Key resources table

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Genetic reagent (Mus musculus)	Prrx1^Cre	The Jackson Laboratory	Strain #: 005584; RRID:IMSR_JAX:005584
Genetic reagent (M. musculus)	Smn1^f7/+	The Jackson Laboratory	Strain #: 006138; RRID:IMSR_JAX:006138
Genetic reagent (M. musculus)	Rosa26^LSL-YFP/+	The Jackson Laboratory	Strain #: 006148; RRID:IMSR_JAX:006148
Genetic reagent (M. musculus)	Rosa26^{LSL-tdTomato/+}	The Jackson Laboratory	Strain #: 007914; RRID:IMSR_JAX:007914
Genetic reagent (M. musculus)	SMN2^+/+; Smn1^+/−	The Jackson Laboratory	Strain #: 005024; RRID:IMSR_JAX:005024
Genetic reagent (M. musculus)	Smn1^+/−; SMN2^+/+; SMNΔ7^+/+	The Jackson Laboratory	Strain #: 005025; RRID:IMSR_JAX:005025
Antibody	anti-SMN (Mouse monoclonal)	BD Biosciences	Cat. #: 610646; RRID:AB_397973	WB (1:1000)
Antibody	anti-alpha-tubulin (Rabbit monoclonal)	Abcam	Cat. #: ab176560; RRID:AB_2860019	WB (1:1000)
Antibody	anti-Neurofilament M (Rabbit polyclonal)	Merckmillipore	Cat. #: ab1987; RRID:AB_91201	IF (1:1000)
Antibody	anti-Synaptophysin 1 (Guinea pig polyclonal)	Synaptic Systems	Cat. #: 101 004; RRID:AB_1210382	IF (1:500)
Antibody	anti-GFP (Chicken polyclonal)	Abcam	Cat. #: ab13970; RRID:AB_300798	IF (1:500)
Antibody	anti-Ki67 (Rabbit polyclonal)	Abcam	Cat. #: ab15580; RRID:AB_443209	IF (1:500)
Antibody	anti-Itgb3 (Rabbit monoclonal)	Cell Signaling	Cat. #: 13166; RRID:AB_2798136	IF (1:100)
Antibody	anti-p-AKT(S473) (Rabbit monoclonal)	Cell Signaling	Cat. #: 4060; RRID:AB_2315049	IF (1:100)
Antibody	anti-CD45-APC (Rat monoclonal)	Biolegend	Cat. #: 103111; RRID:AB_312976	FACS (3 µl per test)
Antibody	anti-CD31-APC (Rat monoclonal)	Biolegend	Cat. #: 102409; RRID:AB_312904	FACS (3 µl per test)
Antibody	anti-Sca-1(Ly6a)-FITC (Rat monoclonal)	Biolegend	Cat. #: 122507; RRID:AB_756192	FACS (3 µl per test)
Antibody	anti-Sca-1(Ly6a)-Pacific blue (Rat monoclonal)	Biolegend	Cat. #: 108120; RRID:AB_493273	FACS (3 µl per test)
Antibody	anti-Vcam1-Biotin (Rat monoclonal)	Biolegend	Cat. #: 105703; RRID:AB_313204	FACS (3 µl per test)
Antibody	anti-Rabbit IgG-HRP (Goat monoclonal)	Promega	Cat. #: W4011; RRID:AB_430833	WB (1:10,000)
Antibody	anti-Mouse IgG-HRP (Goat monoclonal)	Promega	Cat. #: W4021; RRID:AB_430834	WB (1:10,000)
Antibody	anti-Rabbit IgG-Alexa fluor 488 (Goat monoclonal)	Invitrogen	Cat. #: A11034; RRID:AB_2576217	IF (1:500)
Antibody	anti-Chicken IgY-Alexa fluor 488 (Goat monoclonal)	Invitrogen	Cat. #: A11039; RRID:AB_2534096	IF (1:500)
Antibody	anti-Rabbit IgG-Alexa fluor Plus 647 (Goat monoclonal)	Invitrogen	Cat. #: A32733; RRID:AB_2633282	IF (1:500)
Antibody	anti-Guine pig IgG-Alexa fluor 405 (Goat monoclonal)	Abcam	Cat. #: ab175678; RRID:AB_2827755	IF (1:500)
Peptide, recombinant protein	PE-Cy7-Streptavidin	Biolegend	Cat. #: 405206	FACS (3 µl per test)
Peptide, recombinant protein	Alpha-bungarotoxin-Alexa fluor 555	Invitrogen	Cat. #: B35451	IF (1:1000)
Peptide, recombinant protein	Alpha-bungarotoxin-Alexa fluor 488	Invitrogen	Cat. #: B13422	IF (1:1000)
Peptide, recombinant protein	μ-conotoxin GIIIB	Alomone	Cat. #: C-270	Electrophysiology (2.5 µM)
Commercial assay or kit	Pierce BCA protein assay kits	Thermo Fisher Scientific	Cat. #: C-23225
Software	ImageJ	ImageJ	RRID:SCR_003070
Software	AccuCT	PerkinElmer
Software	Microsoft Excel	Microsoft	RRID:SCR_016137
Software	Leica Application Suite X	Leica	RRID:SCR_013673
Software	GraphPad Prism	Graphpad	RRID:SCR_002798
Software	pClamp	Molecular devices	RRID:SCR_011323
Software	QIAGEN	QIAGEN	RRID:SCR_008539
Other	Gill No. 3 formula hematoxylin	Sigma	Cat. #: GHS332	Hematoxylin staining solution (1×)
Other	Eosin Y solution	Sigma	Cat. #: HT110116	Eosin staining solution (1×)
Other	Toluidine blue	Sigma	Cat. #: 198161	Toluidine blue staining (1%)

Animals

Prrx1^Cre (stock 005584), Smn1^f7/+ (stock 006138), Rosa26 ^LSL-YFP/+ (stock 006148), Rosa26 ^{LSL-tdTomato/+} (stock 007914), SMN2^+/+, Smn1^+/− (stock 005024 – Smn1 knockout and SMN2 homologous transgenic mouse) and Smn1^+/−; SMN2^+/+; SMNΔ7^+/+ (stock 005025 005024 – Smn1 knockout and SMN2, SMNΔ7 homologous transgenic mouse) mice were acquired from The Jackson Laboratory (Bar Harbor, ME, USA). SMN2 0-copy Smn1^ΔMPC mice (Prrx1^Cre; Smn1^f7/f7), SMN2 1-copy Smn1^ΔMPC mice (Prrx1^Cre; Smn1^f7/f7; SMN2^+/0 – SMN2 heterologous allele), and SMN2 2-copy Smn1^ΔMPC mice (Prrx1^Cre; Smn1^f7/f7; SMN2^+/+ – SMN2 homologous allele) were generated by crossing Prrx1^Cre mice with Smn1^f7/+ and Smn1^+/−; SMN2^+/+mice. To utilize FAPs transplantation, we generated Prrx1^Cre; Rosa26 ^LSL-YFP/+ mice and Prrx1^Cre; Rosa26 ^{LSL-tdTomato/+} mice by breeding Prrx1^Cre with Rosa26 ^LSL-YFP/+ and Rosa26 ^{LSL-tdTomato/+} mice, respectively. To avoid deletion of the floxed allele by Prrx1^Cre expression in female germline, we bred females without Prrx1^Cre line to Prrx1^Cre transgenic males. SMAΔ7 mutants (Smn1^−/−; SMN2^+/+; SMNΔ7^+/+) were produced by mating Smn1^+/−; SMN2^+/+; SMNΔ7^+/+ mice. Both male and female mice were used in the experiments, and no sex-specific differences were observed. Control littermates lacking Prrx1^Cre were utilized for analysis. All mouse lines were housed under controlled conditions with specific pathogen free and handled according to the guidelines of the Seoul National University Institutional Animal Care and Use Committee (Protocol number: SNU-210313-1).

Micro-CT

The femurs from three groups – control, SMN2 2-copy Smn1^ΔMPC, and SMN2 1-copy Smn1^ΔMPC mice at postnatal day 14 (P14) – were isolated and cleaned of muscles and skin. Subsequently, the femurs were preserved in 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS) overnight at 4°C before micro-CT. The femurs were then imaged through a Quantum GX II micro-CT imaging system (PerkinElmer, Hopkinton, MA, USA). The X-ray source for scanning was set at 90 kV and 88 mA with a field of view of 10 mm (voxel size, 20 μm; scanning time, 14 min). The 3D imaging was viewed using the 3D Viewer software of the Quantum GX II. The size and volume of the femur bone were measured via AccuCT analysis software within the ossified diaphysis and metaphysis of the femur, excluding the epiphysis. BMD calibration was performed using a 4.5-mm BMD phantom and BMD measurements were taken at the center of the diaphysis.

Histology

Alcian blue and alizarin red staining was performed on control and SMN2 0-copy Smn1^ΔMPC mice at E18.5 using a previously reported protocol (Ovchinnikov, 2009). For H&E and toluidine blue staining of the growth plate in the control and SMN2 1-copy Smn1^ΔMPC mice at P14, the femurs were fixed in 4% PFA for 24 hr, rinsed in running tap water for 24 hr, and incubated with 10% ethylenediaminetetraacetic acid (EDTA) (pH 7.4) at 4°C with shaking for 2–3 days. Subsequently, the samples were rinsed in running tap water for 24 hr and then dehydrated through ethanol/xylene and embedded in paraffin. The embedded samples were then sectioned to a thickness of 5 µm, rehydrated, and stained with Gill No. 3 formula hematoxylin and eosin Y (H&E, Sigma-Aldrich, St. Louis, MO, USA) and toluidine blue (Sigma-Aldrich, St. Louis, MO, USA). Stained slides were analyzed using ×10 and ×20 objectives in the EVOS M7000 imaging system (Thermo Fisher Scientific, Waltham, MA, USA). The growth plate’s proliferative and hypertrophic zones were defined by their respective cell sizes.

Hindlimb FAPs isolation

Isolation of limb muscle FAPs was performed according to a previously reported protocol (Kim et al., 2020) with modifications. Limb muscles were dissected and mechanically dissociated in Dulbecco’s modified Eagle’s medium (DMEM, Hyclone) containing 10% horse serum (Hyclone, Logan, UT, USA), collagenase II (800 units/ml; Worthington, Lakewood, NJ, USA), and dispase (1.1 units/ml; Thermo Fisher Scientific, Waltham, MA, USA) at 37°C for 40 min. Digested suspensions were subsequently triturated by sterilized syringes with 20 G 1/2 needle (BD Biosciences, Franklin Lakes, NJ, USA) and washed with DMEM to harvest mononuclear cells. Mononuclear cells were stained with corresponding antibodies. All antibodies used in fluorescence-activated cell sorting (FACS) analysis are listed in Supplementary file 1. To exclude dead cells, 7AAD (Sigma-Aldrich; St. Louis, MO, USA) was used. Stained cells were analyzed and 7AAD⁻Lin⁻Vcam⁻Sca1⁺ (stem cell antigen 1; Ly6a) (FAPs) were isolated using FACS Aria III cell sorter (BD Biosciences) with four-way purity precision. For western blot, freshly isolated FAPs were cultured at 37°C in alpha-MEM (Hyclone) supplemented with Antibiotic–Antimycotic (anti–anti, Gibco) and 20% fetal bovine serum (FBS; Hyclone). For transplantation, YFP⁺ FAPs and tdTomato⁺ FAPs were isolated from postnatal day 10–21 Prrx1^Cre; Rosa26 ^LSL-YFP/+ mice and Prrx1^Cre; Rosa26 ^{LSL-tdTomato/+} mice, respectively. Isotype control density plots were used as a reference for positive gating.

Chondrocytes isolation

Isolation of chondrocytes followed a modified protocol previously published (Jonason et al., 2015). Using blunt forceps, cartilage caps were removed from P21 mouse femoral heads and dissected into ~1 mm fragments in a Petri dish with 10× anti–anti in PBS. The cartilage fragments were washed twice with PBS and then incubated in 5 ml collagenase II solution (800 units/mL collagenase II in DMEM with anti–anti, sterilized by 0.2 µm filtration) in a 60 mm culture dish at 37°C in a 5% CO₂ incubator overnight. Chondrocytes were released by pipetting the remaining cartilage fragments 10 times and then filtering them through a 70-μm cell strainer to a 50-ml conical tube. The cells were then washed twice with PBS and pelleted by centrifugation at 500 × g for 5 min. Subsequently, the cells were cultured overnight at 37°C in a 5% CO₂ incubator, in complete culture medium (DMEM with 10% FBS and anti–anti) overnight at 37°C in a 5% CO₂ incubator.

PCR reaction

To detect the presence of Smn1 exon 7 floxed and deleted allele, 20 mg of each tissue were dissolved in direct PCR buffer (VIAGEN) with proteinase K overnight at 65°C. After inactivation at 95°C for 30 min, PCR was performed using previously reported primer sets to verify the existence of Cre, Smn1^f7, and Smn1^Δ7 alleles (Frugier et al., 2000). Primers are listed in .

RNA extraction and measurement of mRNA expression

Total RNA was extracted from the brain, liver, spinal cord L4, tibialis anterior muscle, freshly isolated FAPs, and chondrocytes using a TRIzol Reagent (Life Technologies, Carlsbad, CA, USA) and analyzed by qRT-PCR. First-strand complementary DNA was synthesized from 1 μg of RNA using ReverTra Ace (Toyobo, Osaka, Japan) containing random oligomer according to the manufacturer’s instructions. qRT-PCR (QIAGEN) was performed with SYBR Green technology (SYBR Premix Ex Taq, QIAGEN) using specific primers against indicated genes. Relative mRNA levels were determined using the 2^−ΔΔCt method and normalized to Gapdh (Figure 1H, Figure 1—figure supplement 1K). Primers are listed in Supplementary file 1.

Western blot

Cultured FAPs at passage 3 were homogenized in radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris–HCl, pH 7.5, 0.5% sodium dodecyl sulfate, 20 µg/ml aprotinin, 20 µg/ml leupeptin, 10 µg/ml phenylmethylsulfonyl fluoride, 1 mM sodium orthovanadate, 10 mM sodium pyrophosphate, 10 mM sodium fluoride, and 1 mM dithiothreitol). Cell lysates were centrifuged at 13,000 rpm for 15 min. Supernatants were collected and subjected to immunoblot. BCA protein assay (Thermo Fisher Scientific) was used for estimating total protein concentrations. Normalized total proteins were analyzed by electrophoresis in 10% polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). Membranes were blocked in 5% skim milk (BD Biosciences) in tris-buffered saline (TBS) with 0.1% Tween-20 and incubated with primary antibodies overnight at 4°C. After incubation with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies, the membranes were developed using a Fusion solo chemiluminescence imaging system (Vilber, Marne-la-Vallée, France). α-Tubulin was used as a loading control. Band intensities were quantified using ImageJ software. Antibodies used in this study are listed in Supplementary file 1. Primary and secondary antibodies were diluted 1:1000 and 1:10,000 with PBS containing 0.1% Tween-20 and 3% bovine serum albumin, respectively.

FAPs transplantation

FAPs transplantation was performed according to a previously reported protocol (Kim et al., 2020) with modifications. YFP⁺ or tdTomato⁺ FAPs (7AAD⁻Lin⁻Vcam⁻Sca1⁺) were isolated by FACS from the limb muscles of indicated mice. 1 × 10⁵ FAPs were suspended in 0.1% gelatin (Sigma-Aldrich, St. Louis, MO, USA) in PBS and then transplanted into one side of the TA muscles of SMN2 1-copy Smn1^ΔMPC mice. The contralateral muscle received an equivalent volume of 0.1% gelatin in PBS (Vehicle).

Electrophysiology

The EDL muscle was dissected from control and SMN2 1-copy Smn1^ΔMPC mice, along with the peroneal nerve, and then pinned to a Sylgard-coated recording chamber. Intracellular recording was conducted in oxygenated Ringer’s solution, which comprised 138.8 mM NaCl, 4 mM KCl, 12 mM NaHCO₃, 1 mM KH₂PO₄, 1 mM MgCl₂, and 2 mM CaCl₂ with a pH of 7.4. Action potential of the muscle was prevented by preparing the muscle in 2.5 μM μ-conotoxin GIIIB (Alomone, Jerusalem, Israel) for 10 min beforehand. The recording was performed in toxin-free Ringer’s solution. mEPPs were recorded from a junction, followed by recordings of eEPPs by stimulating the attached peroneal nerve. The eEPPs were elicited using evoked stimulation. Paired-pulse stimulation (10-ms interstimulus interval) was utilized to assess synaptic transmission. The data were obtained and analyzed with Axoclamp 900A and Clampfit version 10.7 software.

Transmission electron microscopy

NMJ TEM followed a modified protocol previously reported (Modla et al., 2010). Mouse EDL muscle was swiftly excised and fixed in 4% PFA dissolved in Sorensen’s phosphate buffer (0.1 M, pH 7.2), followed by washing in 0.1 M phosphate buffer. The EDL was then gradually infiltrated on a rotator at room temperature with sucrose: 0.1 M phosphate buffer solutions of 30% and 50%, for 1 hr each, followed by an overnight incubation in 70% sucrose. Excess sucrose was then eliminated using filter paper, and the muscle was embedded in an optimal cutting temperature compound (O.C.T.; Sakura Fineteck, Torrance, CA, USA), followed by being frozen in a cryostat (Leica, Wetzlar, Germany). 10-µm-thick longitudinal sections were washed in PBS and treated with Alexa fluor 555-conjugated α-bungarotoxin (1:500, Invitrogen) for an hour. Imaging was conducted with the EVOS M7000 imaging system, and we selected four to five NMJ-rich regions for processing with TEM. The sections were fixed with 2% glutaraldehyde and 2% PFA in 0.1 M cacodylate buffer (pH 7.2) for 2 hr at room temperature, with an additional overnight incubation at 4°C. After washing with 0.1 M cacodylate buffer they were post-fixed with 1% osmium tetraoxide in 0.1 M cacodylate buffer (pH 7.2) for 2 hr at 4°C. The sections were then stained en bloc with 0.5% uranyl acetate overnight, washed with distilled water, and dehydrated using serial ethanol and propylene oxide. The sections were embedded in epoxy resin (Embed-812, Electron Microscopy Sciences) and detached from the slides by dipping them in liquid nitrogen. Ultra-thin sections (70 nm) were prepared with a diamond knife on an ultramicrotome (ULTRACUT UC7, Leica) and mounted on 100 mesh copper grids. Sections were stained with 2% uranyl acetate for 10 min and lead citrate for 3 min, then observed using a transmission electron microscope (80 kV, JEM1010, JEOL or 120 kV, Talos L120C, FEI). Synaptic vesicle density was quantified within a distance of 500 nm from the presynaptic membrane.

Immunohistochemistry

For NMJ staining, freshly dissected TA muscles were fixed in 4% PFA for 30 min at room temperature. Subsequently, the muscles were cryoprotected in 30% sucrose overnight, embedded in O.C.T., snap-frozen in liquid nitrogen, and stored at −80°C prior to sectioning. Longitudinal 40-μm-thick sections were obtained from the embedded muscles using a cryostat. The sections were blocked for 2 hr at room temperature using 5% goat serum and 5% bovine serum albumin in PBS/0.4% Triton X-100. Then, the sections were incubated with primary antibodies in the blocking buffer for 2 days at 4°C. After washing the sections three times with PBS/0.4% Triton X-100, the sections were stained with secondary antibodies overnight at 4°C, and then incubated with Alexa fluor 488- or 555-conjugated α-bungarotoxin (1:500, Invitrogen) for 2 hr at room temperature (RT), washed three times with PBS/0.4% Triton X-100 and mounted in VECTASHIELD. Z-serial images were collected at ×40 with a Leica SP8 confocal laser scanning microscope. To analyze NMJ morphology, LasX software was used to obtain maximal projections. NF varicosity refers to the varicose NF end connected to the rest of the nerve terminal. To quantify NMJ size and synaptophysin coverage, the Btx area and synaptophysin area were measured by ImageJ analysis software.

For bone section staining, the 5-µm-thick bone sections were rehydrated and antigen retrieval was then performed in citrate buffer (10 mM citric acid, pH 6) at 95°C 20 min. The sections were blocked for 1 hr at room temperature using 5% goat serum and 5% bovine serum albumin in PBS/0.4% Triton X-100. Then, the sections were incubated with primary antibodies in the blocking buffer at 4°C overnight. After washing the sections three times with PBS/0.1% Triton X-100, the sections were stained with secondary antibodies for 1 hr at RT, washed and mounted. Imaging was conducted with the EVOS M7000 imaging system.

Statistical analysis

Sample size determination was based on anticipated variability and effect size that was observed in the investigator’s lab for similar experiments. For quantification, individual performing the counts were blinded to sample identity and randomized. All statistical analyses were performed using GraphPad Prism 9 (GraphPad Software). For comparison of significant differences in multiple groups for normally distributed data, statistical analysis was performed by one- or two-way analysis of variance (ANOVA) followed by Tukey’s pairwise comparison post hoc test. For non-normally distributed data, Brown–Forsythe and Welch ANOVA followed by Games–Howell multiple comparisons test was used. For the comparison of two groups, Student’s unpaired t-test assuming a two-tailed distribution with Welch’s correction was used. Unless otherwise noted, all error bars represent standard error of the mean. The number of biological replicates and statistical analyses for each experiment were indicated in the figure legends. Independent experiments were performed at least in triplicates. p < 0.05 was considered statistically significant at the 95% confidence level. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Data availability

All data generated or analyzed during this study are included in the manuscript and supporting files; source data files have been provided for Figure 2.

References

1. Bachman JF
2. Chakkalakal JV
(2022) Insights into muscle stem cell dynamics during postnatal development
The FEBS Journal 289:2710–2722.
https://doi.org/10.1111/febs.15856
- PubMed
- Google Scholar
(1993)
Role of insulin-like growth factors in embryonic and postnatal growth

Cell 75:73–82.
- PubMed
- Google Scholar
1. Baranello G
2. Vai S
3. Broggi F
4. Masson R
5. Arnoldi MT
6. Zanin R
7. Mastella C
8. Bianchi ML
(2019) Evolution of bone mineral density, bone metabolism and fragility fractures in spinal muscular atrophy (SMA) types 2 and 3
Neuromuscular Disorders 29:525–532.
https://doi.org/10.1016/j.nmd.2019.06.001
- PubMed
- Google Scholar
1. Besse A
2. Astord S
3. Marais T
4. Roda M
5. Giroux B
6. Lejeune FX
7. Relaix F
8. Smeriglio P
9. Barkats M
10. Biferi MG
(2020) AAV9-mediated expression of SMN restricted to neurons does not rescue the spinal muscular atrophy phenotype in mice
Molecular Therapy 28:1887–1901.
https://doi.org/10.1016/j.ymthe.2020.05.011
- PubMed
- Google Scholar
1. Bosch-Marcé M
2. Wee CD
3. Martinez TL
4. Lipkes CE
5. Choe DW
6. Kong L
7. Van Meerbeke JP
8. Musarò A
9. Sumner CJ
(2011) Increased IGF-1 in muscle modulates the phenotype of severe SMA mice
Human Molecular Genetics 20:1844–1853.
https://doi.org/10.1093/hmg/ddr067
- PubMed
- Google Scholar
1. Burghes AHM
2. Beattie CE
(2009) Spinal muscular atrophy: why do low levels of survival motor neuron protein make motor neurons sick?
Nature Reviews. Neuroscience 10:597–609.
https://doi.org/10.1038/nrn2670
- PubMed
- Google Scholar
1. Camps J
2. Breuls N
3. Sifrim A
4. Giarratana N
5. Corvelyn M
6. Danti L
7. Grosemans H
8. Vanuytven S
9. Thiry I
10. Belicchi M
11. Meregalli M
12. Platko K
13. MacDonald ME
14. Austin RC
15. Gijsbers R
16. Cossu G
17. Torrente Y
18. Voet T
19. Sampaolesi M
(2020) Interstitial cell remodeling promotes aberrant adipogenesis in dystrophic muscles
Cell Reports 31:107597.
https://doi.org/10.1016/j.celrep.2020.107597
- PubMed
- Google Scholar
(2019) Nusinersen: a treatment for spinal muscular atrophy
The Annals of Pharmacotherapy 53:61–69.
https://doi.org/10.1177/1060028018789956
- PubMed
- Google Scholar
1. Colaianni G
2. Cuscito C
3. Mongelli T
4. Pignataro P
5. Buccoliero C
6. Liu P
7. Lu P
8. Sartini L
9. Di Comite M
10. Mori G
11. Di Benedetto A
12. Brunetti G
13. Yuen T
14. Sun L
15. Reseland JE
16. Colucci S
17. New MI
18. Zaidi M
19. Cinti S
20. Grano M
(2015) The myokine irisin increases cortical bone mass
PNAS 112:12157–12162.
https://doi.org/10.1073/pnas.1516622112
- Google Scholar
(2018) Emerging issues in AAV-mediated in vivo gene therapy
Molecular Therapy - Methods & Clinical Development 8:87–104.
https://doi.org/10.1016/j.omtm.2017.11.007
- Google Scholar
(2021) Origins, potency, and heterogeneity of skeletal muscle fibro-adipogenic progenitors-time for new definitions
Skeletal Muscle 11:16.
https://doi.org/10.1186/s13395-021-00265-6
- PubMed
- Google Scholar
1. Dale JM
2. Shen H
3. Barry DM
4. Garcia VB
5. Rose FF
6. Lorson CL
7. Garcia ML
(2011) The spinal muscular atrophy mouse model, SMAΔ7, displays altered axonal transport without global neurofilament alterations
Acta Neuropathologica 122:331–341.
https://doi.org/10.1007/s00401-011-0848-5
- PubMed
- Google Scholar
1. De Amicis R
2. Baranello G
3. Foppiani A
4. Leone A
5. Battezzati A
6. Bedogni G
7. Ravella S
8. Giaquinto E
9. Mastella C
10. Agosto C
11. Bertini E
12. D’Amico A
13. Pedemonte M
14. Bruno C
15. Wells JC
16. Fewtrell M
17. Bertoli S
(2021) Growth patterns in children with spinal muscular atrophy
Orphanet Journal of Rare Diseases 16:375.
https://doi.org/10.1186/s13023-021-02015-9
- PubMed
- Google Scholar
1. Deguise M
2. Pileggi C
3. Repentigny YD
4. Beauvais A
5. Tierney A
6. Chehade L
7. Michaud J
8. Llavero-hurtado M
9. Lamont D
10. Atrih A
11. Wishart TM
12. Gillingwater TH
13. Schneider BL
14. Harper M
15. Parson SH
16. Kothary R
(2021) SMN depleted mice offer a robust and rapid onset model of nonalcoholic fatty liver disease
Cellular and Molecular Gastroenterology and Hepatology 12:354–377.
https://doi.org/10.1016/j.jcmgh.2021.01.019
- Google Scholar
(2006) Increased and decreased activity elicits specific morphological adaptations of the neuromuscular junction
Neuroscience 137:1277–1283.
https://doi.org/10.1016/j.neuroscience.2005.10.042
- PubMed
- Google Scholar
1. Foust KD
2. Nurre E
3. Montgomery CL
4. Hernandez A
5. Chan CM
6. Kaspar BK
(2009) Intravascular AAV9 preferentially targets neonatal neurons and adult astrocytes
Nature Biotechnology 27:59–65.
https://doi.org/10.1038/nbt.1515
- PubMed
- Google Scholar
1. Frugier T
2. Tiziano FD
3. Cifuentes-Diaz C
4. Miniou P
5. Roblot N
6. Dierich A
7. Le Meur M
8. Melki J
(2000) Nuclear targeting defect of SMN lacking the C-terminus in a mouse model of spinal muscular atrophy
Human Molecular Genetics 9:849–858.
https://doi.org/10.1093/hmg/9.5.849
- PubMed
- Google Scholar
1. Govoni KE
2. Lee SK
3. Chung YS
4. Behringer RR
5. Wergedal JE
6. Baylink DJ
7. Mohan S
(2007) Disruption of insulin-like growth factor-I expression in type IIalphaI collagen-expressing cells reduces bone length and width in mice
Physiological Genomics 30:354–362.
https://doi.org/10.1152/physiolgenomics.00022.2007
- PubMed
- Google Scholar
1. Hallett SA
2. Ono W
3. Ono N
(2019) Growth plate chondrocytes: skeletal development, growth and beyond
International Journal of Molecular Sciences 20:6009.
https://doi.org/10.3390/ijms20236009
- PubMed
- Google Scholar
1. Heller GJ
2. Marshall MS
3. Issa Y
4. Marshall JN
5. Nguyen D
6. Rue E
7. Pathmasiri KC
8. Domowicz MS
9. van Breemen RB
10. Tai LM
11. Cologna SM
12. Crocker SJ
13. Givogri MI
14. Sands MS
15. Bongarzone ER
(2021) Waning efficacy in a long-term AAV-mediated gene therapy study in the murine model of Krabbe disease
Molecular Therapy 29:1883–1902.
https://doi.org/10.1016/j.ymthe.2021.01.026
- PubMed
- Google Scholar
1. Hensel N
2. Brickwedde H
3. Tsaknakis K
4. Grages A
5. Braunschweig L
6. Lüders KA
7. Lorenz HM
8. Lippross S
9. Walter LM
10. Tavassol F
11. Lienenklaus S
12. Neunaber C
13. Claus P
14. Hell AK
(2020) Altered bone development with impaired cartilage formation precedes neuromuscular symptoms in spinal muscular atrophy
Human Molecular Genetics 29:2662–2673.
https://doi.org/10.1093/hmg/ddaa145
- PubMed
- Google Scholar
(2014) Relationship of weight and body mass index with bone mineral density in adult men from kosovo
Materia Socio-Medica 26:306–308.
https://doi.org/10.5455/msm.2014.26.306-308
- PubMed
- Google Scholar
1. Hua Y
2. Sahashi K
3. Rigo F
4. Hung G
5. Horev G
6. Bennett CF
7. Krainer AR
(2011) Peripheral SMN restoration is essential for long-term rescue of a severe spinal muscular atrophy mouse model
Nature 478:123–126.
https://doi.org/10.1038/nature10485
- PubMed
- Google Scholar
1. Hua Y
2. Liu YH
3. Sahashi K
4. Rigo F
5. Bennett CF
6. Krainer AR
(2015) Motor neuron cell-nonautonomous rescue of spinal muscular atrophy phenotypes in mild and severe transgenic mouse models
Genes & Development 29:288–297.
https://doi.org/10.1101/gad.256644.114
- PubMed
- Google Scholar
1. Ike H
2. Inaba Y
3. Kobayashi N
4. Hirata Y
5. Yukizawa Y
6. Aoki C
7. Choe H
8. Saito T
(2015) Comparison between mechanical stress and bone mineral density in the femur after total hip arthroplasty by using subject-specific finite element analyses
Computer Methods in Biomechanics and Biomedical Engineering 18:1056–1065.
https://doi.org/10.1080/10255842.2013.869320
- PubMed
- Google Scholar
Book
(2015) Primary murine growth plate and Articular Chondrocyte isolation and cell culture
In: Westendorf JJ, van Wijnen AJ, editors. Osteoporosis and Osteoarthritis. Humana Press. pp. 11–18.
https://doi.org/10.1007/978-1-4939-1619-1
- Google Scholar
(2011) Genetic regulation of the growth plate
Frontiers in Endocrinology 2:113.
https://doi.org/10.3389/fendo.2011.00113
- PubMed
- Google Scholar
(2008) Reduced SMN protein impairs maturation of the neuromuscular junctions in mouse models of spinal muscular atrophy
Human Molecular Genetics 17:2552–2569.
https://doi.org/10.1093/hmg/ddn156
- PubMed
- Google Scholar
1. Kariya S
2. Obis T
3. Garone C
4. Akay T
5. Sera F
6. Iwata S
7. Homma S
8. Monani UR
(2014) Requirement of enhanced Survival Motoneuron protein imposed during neuromuscular junction maturation
The Journal of Clinical Investigation 124:785–800.
https://doi.org/10.1172/JCI72017
- PubMed
- Google Scholar
(2008) Low bone mineral density in spinal muscular atrophy
Journal of Clinical Neuromuscular Disease 10:11–17.
https://doi.org/10.1097/CND.0b013e318183e0fa
- PubMed
- Google Scholar
1. Kim JK
2. Jha NN
3. Feng Z
4. Faleiro MR
5. Chiriboga CA
6. Wei-Lapierre L
7. Dirksen RT
8. Ko CP
9. Monani UR
(2020) Muscle-specific SMN reduction reveals motor neuron-independent disease in spinal muscular atrophy models
The Journal of Clinical Investigation 130:1271–1287.
https://doi.org/10.1172/JCI131989
- PubMed
- Google Scholar
1. Kim JH
2. Kang JS
3. Yoo K
4. Jeong J
5. Park I
6. Park JH
7. Rhee J
8. Jeon S
9. Jo YW
10. Hann SH
11. Seo M
12. Moon S
13. Um SJ
14. Seong RH
15. Kong YY
(2022) Bap1/SMN axis in Dpp4+ skeletal muscle mesenchymal cells regulates the neuromuscular system
JCI Insight 7:e158380.
https://doi.org/10.1172/jci.insight.158380
- PubMed
- Google Scholar
(2019) Bone health and growth in spinal muscular atrophy type 2 and 3
Journal of Istanbul Faculty of Medicine / İstanbul Tıp Fakültesi Dergisi 01:22–28.
https://doi.org/10.26650/IUITFD.884235
- Google Scholar
1. Kong L
2. Wang X
3. Choe DW
4. Polley M
5. Burnett BG
6. Bosch-Marcé M
7. Griffin JW
8. Rich MM
9. Sumner CJ
(2009) Impaired synaptic vesicle release and immaturity of neuromuscular junctions in spinal muscular atrophy mice
The Journal of Neuroscience 29:842–851.
https://doi.org/10.1523/JNEUROSCI.4434-08.2009
- PubMed
- Google Scholar
1. Leinroth AP
2. Mirando AJ
3. Rouse D
4. Kobayahsi Y
5. Tata PR
6. Rueckert HE
7. Liao Y
8. Long JT
9. Chakkalakal JV
10. Hilton MJ
(2022) Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations
Cell Reports 39:110785.
https://doi.org/10.1016/j.celrep.2022.110785
- PubMed
- Google Scholar
1. Ling KKY
2. Lin MY
3. Zingg B
4. Feng Z
5. Ko CP
(2010) Synaptic defects in the spinal and neuromuscular circuitry in a mouse model of spinal muscular atrophy
PLOS ONE 5:e15457.
https://doi.org/10.1371/journal.pone.0015457
- PubMed
- Google Scholar
1. Logan M
2. Martin JF
3. Nagy A
4. Lobe C
5. Olson EN
6. Tabin CJ
(2002) Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer
Genesis 33:77–80.
https://doi.org/10.1002/gene.10092
- PubMed
- Google Scholar
1. Lukjanenko L
2. Karaz S
3. Stuelsatz P
4. Gurriaran-Rodriguez U
5. Michaud J
6. Dammone G
7. Sizzano F
8. Mashinchian O
9. Ancel S
10. Migliavacca E
11. Liot S
12. Jacot G
13. Metairon S
14. Raymond F
15. Descombes P
16. Palini A
17. Chazaud B
18. Rudnicki MA
19. Bentzinger CF
20. Feige JN
(2019) Aging disrupts muscle stem cell function by impairing matricellular WISP1 Secretion from fibro-adipogenic progenitors
Cell Stem Cell 24:433–446.
https://doi.org/10.1016/j.stem.2018.12.014
- PubMed
- Google Scholar
1. Martinez TL
2. Kong L
3. Wang X
4. Osborne MA
5. Crowder ME
6. Van Meerbeke JP
7. Xu X
8. Davis C
9. Wooley J
10. Goldhamer DJ
11. Lutz CM
12. Rich MM
13. Sumner CJ
(2012) Survival motor neuron protein in motor neurons determines synaptic integrity in spinal muscular atrophy
The Journal of Neuroscience 32:8703–8715.
https://doi.org/10.1523/JNEUROSCI.0204-12.2012
- PubMed
- Google Scholar
1. Mattar CN
2. Waddington SN
3. Biswas A
4. Johana N
5. Ng XW
6. Fisk AS
7. Fisk NM
8. Tan LG
9. Rahim AA
10. Buckley SMK
11. Tan MH
12. Lu J
13. Choolani M
14. Chan JKY
(2013) Systemic delivery of scAAV9 in fetal macaques facilitates neuronal transduction of the central and peripheral nervous systems
Gene Therapy 20:69–83.
https://doi.org/10.1038/gt.2011.216
- PubMed
- Google Scholar
1. McGovern VL
2. Iyer CC
3. Arnold WD
4. Gombash SE
5. Zaworski PG
6. Blatnik AJ
7. Foust KD
8. Burghes AHM
(2015) SMN expression is required in motor neurons to rescue electrophysiological deficits in the SMNΔ7 mouse model of SMA
Human Molecular Genetics 24:5524–5541.
https://doi.org/10.1093/hmg/ddv283
- PubMed
- Google Scholar
1. Mercuri E
2. Pera MC
3. Scoto M
4. Finkel R
5. Muntoni F
(2020) Spinal muscular atrophy - insights and challenges in the treatment era
Nature Reviews. Neurology 16:706–715.
https://doi.org/10.1038/s41582-020-00413-4
- PubMed
- Google Scholar
1. Mercuri E
2. Sumner CJ
3. Muntoni F
4. Darras BT
5. Finkel RS
(2022) Spinal muscular atrophy
Nature Reviews. Disease Primers 8:.
https://doi.org/10.1038/s41572-022-00380-8
- PubMed
- Google Scholar
(2010) Identification of neuromuscular junctions by correlative confocal and transmission electron microscopy
Journal of Neuroscience Methods 191:158–165.
https://doi.org/10.1016/j.jneumeth.2010.06.015
- PubMed
- Google Scholar
1. Monani UR
2. Sendtner M
3. Coovert DD
4. Parsons DW
5. Andreassi C
6. Le TT
7. Jablonka S
8. Schrank B
9. Rossoll W
10. Prior TW
11. Morris GE
12. Burghes AH
(2000) The human centromeric survival motor neuron gene (SMN2) rescues embryonic lethality in Smn(-/-) mice and results in a mouse with spinal muscular atrophy
Human Molecular Genetics 9:333–339.
https://doi.org/10.1093/hmg/9.3.333
- PubMed
- Google Scholar
1. Monani UR
2. Pastore MT
3. Gavrilina TO
4. Jablonka S
5. Le TT
6. Andreassi C
7. DiCocco JM
8. Lorson C
9. Androphy EJ
10. Sendtner M
11. Podell M
12. Burghes AHM
(2003) A transgene carrying an A2G missense mutation in the SMN gene modulates phenotypic severity in mice with severe (type I) spinal muscular atrophy
The Journal of Cell Biology 160:41–52.
https://doi.org/10.1083/jcb.200208079
- PubMed
- Google Scholar
(2012) IPLEX administration improves motor neuron survival and ameliorates motor functions in a severe mouse model of spinal muscular atrophy
Molecular Medicine 18:1076–1085.
https://doi.org/10.2119/molmed.2012.00056
- PubMed
- Google Scholar
(2017) Non-myogenic contribution to muscle development and homeostasis: the role of connective tissues
Frontiers in Cell and Developmental Biology 5:22.
https://doi.org/10.3389/fcell.2017.00022
- PubMed
- Google Scholar
1. Oichi T
2. Kodama J
3. Wilson K
4. Tian H
5. Imamura Kawasawa Y
6. Usami Y
7. Oshima Y
8. Saito T
9. Tanaka S
10. Iwamoto M
11. Otsuru S
12. Enomoto-Iwamoto M
(2023) Nutrient-regulated dynamics of chondroprogenitors in the postnatal murine growth plate
Bone Research 11:20.
https://doi.org/10.1038/s41413-023-00258-9
- PubMed
- Google Scholar
1. Ovchinnikov D
(2009) Alcian blue/alizarin red staining of cartilage and bone in mouse
Cold Spring Harbor Protocols 2009:pdb.prot5170.
https://doi.org/10.1101/pdb.prot5170
- PubMed
- Google Scholar
(2010) Reduced survival of motor neuron (SMN) protein in motor neuronal progenitors functions cell autonomously to cause spinal muscular atrophy in model mice expressing the human centromeric (SMN2) gene
The Journal of Neuroscience 30:12005–12019.
https://doi.org/10.1523/JNEUROSCI.2208-10.2010
- PubMed
- Google Scholar
(2010) CNS-targeted gene therapy improves survival and motor function in a mouse model of spinal muscular atrophy
The Journal of Clinical Investigation 120:1253–1264.
https://doi.org/10.1172/JCI41615
- PubMed
- Google Scholar
1. Passini MA
2. Bu J
3. Richards AM
4. Kinnecom C
5. Sardi SP
6. Stanek LM
7. Hua Y
8. Rigo F
9. Matson J
10. Hung G
11. Kaye EM
12. Shihabuddin LS
13. Krainer AR
14. Bennett CF
15. Cheng SH
(2011) Antisense oligonucleotides delivered to the mouse CNS ameliorate symptoms of severe spinal muscular atrophy
Science Translational Medicine 3:1–12.
https://doi.org/10.1126/scitranslmed.3001777
- PubMed
- Google Scholar
(2008) Adeno-associated virus vector genomes persist as episomal chromatin in primate muscle
Journal of Virology 82:7875–7885.
https://doi.org/10.1128/JVI.00649-08
- PubMed
- Google Scholar
1. Petrany MJ
2. Swoboda CO
3. Sun C
4. Chetal K
5. Chen X
6. Weirauch MT
7. Salomonis N
8. Millay DP
(2020) Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers
Nature Communications 11:6374.
https://doi.org/10.1038/s41467-020-20063-w
- PubMed
- Google Scholar
1. Racine HL
2. Serrat MA
(2020) The actions of IGF-1 in the growth plate and its role in postnatal bone elongation
Current Osteoporosis Reports 18:210–227.
https://doi.org/10.1007/s11914-020-00570-x
- PubMed
- Google Scholar
1. Romeo SG
2. Alawi KM
3. Rodrigues J
4. Singh A
5. Kusumbe AP
6. Ramasamy SK
(2019) Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation
Nature Cell Biology 21:430–441.
https://doi.org/10.1038/s41556-019-0304-7
- PubMed
- Google Scholar
1. Ross JA
2. Webster RG
3. Lechertier T
4. Reynolds LE
5. Turmaine M
6. Bencze M
7. Jamshidi Y
8. Cetin H
9. Muntoni F
10. Beeson D
11. Hodilvala-Dilke K
12. Conti FJ
(2017) Multiple roles of integrin-α3 at the neuromuscular junction
Journal of Cell Science 130:1772–1784.
https://doi.org/10.1242/jcs.201103
- PubMed
- Google Scholar
1. Sahashi K
2. Ling KKY
3. Hua Y
4. Wilkinson JE
5. Nomakuchi T
6. Rigo F
7. Hung G
8. Xu D
9. Jiang YP
10. Lin RZ
11. Ko CP
12. Bennett CF
13. Krainer AR
(2013) Pathological impact of SMN2 mis-splicing in adult SMA mice
EMBO Molecular Medicine 5:1586–1601.
https://doi.org/10.1002/emmm.201302567
- PubMed
- Google Scholar
(2019) Hic1 defines quiescent mesenchymal progenitor subpopulations with distinct functions and fates in skeletal muscle regeneration
Cell Stem Cell 25:797–813.
https://doi.org/10.1016/j.stem.2019.11.004
- PubMed
- Google Scholar
1. Sheng G
(2015) The developmental basis of mesenchymal stem/stromal cells (MSCs)
BMC Developmental Biology 15:44.
https://doi.org/10.1186/s12861-015-0094-5
- PubMed
- Google Scholar
1. Shim KS
(2015) Pubertal growth and epiphyseal fusion
Annals of Pediatric Endocrinology & Metabolism 20:8–12.
https://doi.org/10.6065/apem.2015.20.1.8
- PubMed
- Google Scholar
1. Torres-Benito L
2. Neher MF
3. Cano R
4. Ruiz R
5. Tabares L
(2011) SMN requirement for synaptic vesicle, active zone and microtubule postnatal organization in motor nerve terminals
PLOS ONE 6:e26164.
https://doi.org/10.1371/journal.pone.0026164
- PubMed
- Google Scholar
1. Tsai LK
2. Chen CL
3. Ting CH
4. Lin-Chao S
5. Hwu WL
6. Dodge JC
7. Passini MA
8. Cheng SH
(2014) Systemic administration of a recombinant AAV1 vector encoding IGF-1 improves disease manifestations in SMA mice
Molecular Therapy 22:1450–1459.
https://doi.org/10.1038/mt.2014.84
- PubMed
- Google Scholar
1. Uezumi A
2. Ikemoto-Uezumi M
3. Zhou H
4. Kurosawa T
5. Yoshimoto Y
6. Nakatani M
7. Hitachi K
8. Yamaguchi H
9. Wakatsuki S
10. Araki T
11. Morita M
12. Yamada H
13. Toyoda M
14. Kanazawa N
15. Nakazawa T
16. Hino J
17. Fukada SI
18. Tsuchida K
(2021) Mesenchymal Bmp3b expression maintains skeletal muscle integrity and decreases in age-related sarcopenia
The Journal of Clinical Investigation 131:e139617.
https://doi.org/10.1172/JCI139617
- PubMed
- Google Scholar
1. Vai S
2. Bianchi ML
3. Moroni I
4. Mastella C
5. Broggi F
6. Morandi L
7. Arnoldi MT
8. Bussolino C
9. Baranello G
(2015) Bone and spinal muscular atrophy
Bone 79:116–120.
https://doi.org/10.1016/j.bone.2015.05.039
- PubMed
- Google Scholar
1. Valori CF
2. Ning K
3. Wyles M
4. Mead RJ
5. Grierson AJ
6. Shaw PJ
7. Azzouz M
(2010) Systemic delivery of scAAV9 expressing SMN prolongs survival in a model of spinal muscular atrophy
Science Translational Medicine 2:35ra42.
https://doi.org/10.1126/scitranslmed.3000830
- PubMed
- Google Scholar
(2021) Gain of toxic function by long-term AAV9-mediated SMN overexpression in the sensorimotor circuit
Nature Neuroscience 24:930–940.
https://doi.org/10.1038/s41593-021-00827-3
- PubMed
- Google Scholar
1. Wang J
2. Zhou J
3. Bondy CA
(1999) Igf1 promotes longitudinal bone growth by insulin-like actions augmenting chondrocyte hypertrophy
FASEB Journal 13:1985–1990.
https://doi.org/10.1096/fasebj.13.14.1985
- PubMed
- Google Scholar
1. Wang Y
2. Cheng Z
3. Elalieh HZ
4. Nakamura E
5. Nguyen MT
6. Mackem S
7. Clemens TL
8. Bikle DD
9. Chang W
(2011) IGF-1R signaling in chondrocytes modulates growth plate development by interacting with the PTHrP/Ihh pathway
Journal of Bone and Mineral Research 26:1437–1446.
https://doi.org/10.1002/jbmr.359
- PubMed
- Google Scholar
1. Wasserman HM
2. Hornung LN
3. Stenger PJ
4. Rutter MM
5. Wong BL
6. Rybalsky I
7. Khoury JC
8. Kalkwarf HJ
(2017) Low bone mineral density and fractures are highly prevalent in pediatric patients with spinal muscular atrophy regardless of disease severity
Neuromuscular Disorders 27:331–337.
https://doi.org/10.1016/j.nmd.2017.01.019
- PubMed
- Google Scholar
1. Wilk K
2. Yeh SCA
3. Mortensen LJ
4. Ghaffarigarakani S
5. Lombardo CM
6. Bassir SH
7. Aldawood ZA
8. Lin CP
9. Intini G
(2017) Postnatal calvarial skeletal stem cells expressing PRX1 reside exclusively in the calvarial sutures and are required for bone regeneration
Stem Cell Reports 8:933–946.
https://doi.org/10.1016/j.stemcr.2017.03.002
- PubMed
- Google Scholar
1. Wosczyna MN
2. Konishi CT
3. Perez Carbajal EE
4. Wang TT
5. Walsh RA
6. Gan Q
7. Wagner MW
8. Rando TA
(2019) Mesenchymal stromal cells are required for regeneration and homeostatic maintenance of skeletal muscle
Cell Reports 27:2029–2035.
https://doi.org/10.1016/j.celrep.2019.04.074
- PubMed
- Google Scholar
1. Yakar S
2. Liu JL
3. Stannard B
4. Butler A
5. Accili D
6. Sauer B
7. LeRoith D
(1999) Normal growth and development in the absence of hepatic insulin-like growth factor I
PNAS 96:7324–7329.
https://doi.org/10.1073/pnas.96.13.7324
- PubMed
- Google Scholar
1. Yakar S
2. Rosen CJ
3. Beamer WG
4. Ackert-Bicknell CL
5. Wu Y
6. Liu JL
7. Ooi GT
8. Setser J
9. Frystyk J
10. Boisclair YR
11. LeRoith D
(2002) Circulating levels of IGF-1 directly regulate bone growth and density
The Journal of Clinical Investigation 110:771–781.
https://doi.org/10.1172/JCI15463
- PubMed
- Google Scholar
1. Yang YS
2. Xie J
3. Wang D
4. Kim JM
5. Tai PWL
6. Gravallese E
7. Gao G
8. Shim JH
(2019) Bone-targeting AAV-mediated silencing of Schnurri-3 prevents bone loss in osteoporosis
Nature Communications 10:2958.
https://doi.org/10.1038/s41467-019-10809-6
- PubMed
- Google Scholar
(2016) Serum IGF1 and IGFBP3 levels in SMA patients
Neuromuscular Disorders 26:S105.
https://doi.org/10.1016/j.nmd.2016.06.075
- Google Scholar
(2018) Electrophysiological recordings of evoked end-plate potential on murine neuro-muscular synapse preparations
BIO-PROTOCOL 8:e2803.
https://doi.org/10.21769/BioProtoc.2803
- PubMed
- Google Scholar
1. Zhang Z
2. Lotti F
3. Dittmar K
4. Younis I
5. Wan L
6. Kasim M
7. Dreyfuss G
(2008) SMN deficiency causes tissue-specific perturbations in the repertoire of snRNAs and widespread defects in splicing
Cell 133:585–600.
https://doi.org/10.1016/j.cell.2008.03.031
- PubMed
- Google Scholar

Article and author information

Author details

Sang-Hyeon Hann

School of Biological Sciences, Seoul National University, Seoul, Republic of Korea

Contribution
Conceptualization, Data curation, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing – original draft, Writing – review and editing

Contributed equally with
Seon-Yong Kim

Competing interests
No competing interests declared

"This ORCID iD identifies the author of this article:" 0000-0002-0871-3414
Seon-Yong Kim

Department of Physiology, Dental Research Institute, Seoul National University School of Dentistry, Seoul, Republic of Korea

Contribution
Data curation, Formal analysis, Validation, Investigation, Methodology, Writing – review and editing

Contributed equally with
Sang-Hyeon Hann

Competing interests
No competing interests declared
Ye Lynne Kim

School of Biological Sciences, Seoul National University, Seoul, Republic of Korea

Contribution
Validation, Investigation, Writing – review and editing

Competing interests
No competing interests declared
Young-Woo Jo

School of Biological Sciences, Seoul National University, Seoul, Republic of Korea

Contribution
Data curation, Formal analysis, Methodology, Writing – review and editing

Competing interests
No competing interests declared
Jong-Seol Kang

School of Biological Sciences, Seoul National University, Seoul, Republic of Korea

Contribution
Formal analysis, Investigation, Methodology

Competing interests
No competing interests declared
Hyerim Park

School of Biological Sciences, Seoul National University, Seoul, Republic of Korea

Contribution
Investigation, Visualization, Methodology

Competing interests
No competing interests declared
Se-Young Choi

Department of Physiology, Dental Research Institute, Seoul National University School of Dentistry, Seoul, Republic of Korea

Contribution
Supervision, Funding acquisition, Methodology, Project administration, Writing – review and editing

For correspondence
sychoi@snu.ac.kr

Competing interests
No competing interests declared

"This ORCID iD identifies the author of this article:" 0000-0001-7534-5167
Young-Yun Kong

School of Biological Sciences, Seoul National University, Seoul, Republic of Korea

Contribution
Supervision, Funding acquisition, Methodology, Project administration, Writing – review and editing

For correspondence
ykong@snu.ac.kr

Competing interests
No competing interests declared

"This ORCID iD identifies the author of this article:" 0000-0001-7335-3729

Funding

National Research Foundation of Korea (NRF-2022R1A2C3007621)

Young-Yun Kong

National Research Foundation of Korea (NRF-2020R1A5A1018081)

Young-Yun Kong

National Research Foundation of Korea (NRF-2020R1A2C3011464)

Se-Young Choi

The funders had no role in study design, data collection, and interpretation, or the decision to submit the work for publication.

Acknowledgements

We express our gratitude to the Kong and Choi laboratory members for their valuable feedback during the project. This work was supported by the National Research Foundation of Korea (NRF-2022R1A2C3007621, NRF-2020R1A5A1018081, and NRF-2020R1A2C3011464).

Ethics

The care and treatment of animals in this study were approved by the Institutional Animal Care and Use Committee (IACUC) protocols (SNU-210313-1) of Seoul National University.

Version history

Sent for peer review: September 21, 2023
Preprint posted: October 10, 2023 (view preprint)
Preprint posted: November 7, 2023 (view preprint)
Preprint posted: January 23, 2024 (view preprint)
Version of Record published: February 6, 2024 (version 1)

Cite all versions

You can cite all versions using the DOI https://doi.org/10.7554/eLife.92731. This DOI represents all versions, and will always resolve to the latest one.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

727

views
106

downloads
0

citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Mendeley

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

Sang-Hyeon Hann
Seon-Yong Kim
Ye Lynne Kim
Young-Woo Jo
Jong-Seol Kang
Hyerim Park
Se-Young Choi
Young-Yun Kong

(2024)

Depletion of SMN protein in mesenchymal progenitors impairs the development of bone and neuromuscular junction in spinal muscular atrophy

eLife 12:RP92731.

https://doi.org/10.7554/eLife.92731.3

Categories and tags

Research organism

Mouse

Share this article

Cite this article

Skeletal growth abnormalities and altered growth plate homeostasis in SMN2 1-copy Smn1ΔMPC mice.

Decreased chondrocyte-derived IGF–AKT axis by limb mesenchymal cell-specific survival motor neuron (SMN) depletion in SMN2 1-copy Smn1ΔMPC mice.

Figure 2—source data 1

Figure 2—source data 2

Aberrant postnatal neuromuscular junction (NMJ) maturation in SMN2 1-copy Smn1ΔMPC mice.

Morphological deterioration in neuromuscular junctions (NMJs) of adult SMN2 1-copy Smn1ΔMPC mice.

Reduced presynaptic neurotransmission ability in the neuromuscular junctions (NMJs) of SMN2 1-copy Smn1ΔMPC mice.

Abnormal nerve terminal ultrastructure in SMN2 1-copy mutant.

Improved postnatal neuromuscular junction (NMJ) development in the TA muscle of SMN2 1-copy Smn1ΔMPC mice following healthy fibro-adipogenic progenitors (FAPs) transplantation.

Author details

Sang-Hyeon Hann

Contribution

Contributed equally with

Competing interests

Seon-Yong Kim

Contribution

Contributed equally with

Competing interests

Ye Lynne Kim

Contribution

Competing interests

Young-Woo Jo

Contribution

Competing interests

Jong-Seol Kang

Contribution

Competing interests

Hyerim Park

Contribution

Competing interests

Se-Young Choi

Contribution

For correspondence

Competing interests

Young-Yun Kong

Contribution

For correspondence

Competing interests

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

Categories and tags

Research organism

Further reading

Skeletal growth abnormalities and altered growth plate homeostasis in SMN2 1-copy Smn1^ΔMPC mice.

Decreased chondrocyte-derived IGF–AKT axis by limb mesenchymal cell-specific survival motor neuron (SMN) depletion in SMN2 1-copy Smn1^ΔMPC mice.

Aberrant postnatal neuromuscular junction (NMJ) maturation in SMN2 1-copy Smn1^ΔMPC mice.

Morphological deterioration in neuromuscular junctions (NMJs) of adult SMN2 1-copy Smn1^ΔMPC mice.

Reduced presynaptic neurotransmission ability in the neuromuscular junctions (NMJs) of SMN2 1-copy Smn1^ΔMPC mice.

Improved postnatal neuromuscular junction (NMJ) development in the TA muscle of SMN2 1-copy Smn1^ΔMPC mice following healthy fibro-adipogenic progenitors (FAPs) transplantation.