A myristoyl switch at the plasma membrane triggers cleavage and oligomerization of Mason-Pfizer monkey virus matrix protein
- University of Chemistry and Technology, Czech Republic
- Czech Academy of Sciences, Czech Republic
Abstract
For most retroviruses, including HIV, association with the plasma membrane (PM) promotes the assembly of immature particles, which occurs simultaneously with budding and maturation. In these viruses, maturation is initiated by oligomerization of polyprotein precursors. In contrast, several retroviruses, such as Mason-Pfizer monkey virus (M-PMV), assemble in the cytoplasm into immature particles that are transported across the PM. Therefore, protease activation and specific cleavage must not occur until the preassembled particle interacts with the PM. This interaction is triggered by a bipartite signal consisting of a cluster of basic residues in the matrix (MA) domain of Gag polyprotein and a myristoyl moiety N-terminally attached to MA. Here, we provide evidence that myristoyl exposure from the MA core and its insertion into the PM occurs in M-PMV. By a combination of experimental methods, we show that this results in a structural change at the C-terminus of MA allowing efficient cleavage of MA from the downstream region of Gag. This suggests that, in addition to the known effect of the myristoyl switch of HIV-1 MA on the multimerization state of Gag and particle assembly, the myristoyl switch may have a regulatory role in initiating sequential cleavage of M-PMV Gag in immature particles.
Data availability
The data were deposited in Dryad under the DOI: https://doi.org/10.5061/dryad.c59zw3rfn
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Data from: A myristoyl switch at the plasma membrane triggers cleavage and oligomerization of Mason-Pfizer monkey virus matrix proteinDryad Digital Repository, doi:10.5061/dryad.c59zw3rfn.
Article and author information
Author details
Funding
Grant agency of the Czech Republic (22-19250S)
- Jan Prchal
Programme Exceles (LX22NPO5103)
- Jan Prchal
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Mauricio Comas-Garcia, Universidad Autónoma de San Luis Potosí, Mexico
Version history
- Received: October 12, 2023
- Preprint posted: December 21, 2023 (view preprint)
- Accepted: March 10, 2024
- Accepted Manuscript published: March 22, 2024 (version 1)
- Accepted Manuscript updated: March 27, 2024 (version 2)
- Version of Record published: April 12, 2024 (version 3)
Copyright
© 2024, Častorálová et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A myristoyl switch at the plasma membrane triggers cleavage and oligomerization of Mason-Pfizer monkey virus matrix protein
eLife 13:e93489.
https://doi.org/10.7554/eLife.93489
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